Studies On Polymorphisms Of H-FABP Gene And The Association With Intramuscular Fat Content In The Goats

With the improvement of living level, mutton becomes peoples' favorite meat owing to its low fat, low cholesterol, high protein and pollution-free. The fat (Intramuscular fat, IMF) content is important to evaluate the quality of muscles. H-FABP is the major gene which influences the precipitation of IMF. The H-FABP polymorphism makes the cells uptake the fatty acids and has important biological functions in the form of meat. Therefore it has a somescitific and practical value for the molecular genetics breeding and marker-assisted selection of the animals when the H-FABP is studied with the production traits as a canditate gene.Direct sequencing was used to find the SNPs at the H-FABP gene in 98 ChongMing white goats,100 ChongMing hybridized goats,91 XuHuai goats and 65 GuanZhong milchgoats from ChongMing Island. PCR-RFLPs were established to differentiate the SNPs; Soxhlet extractor was used to identify the fat content; SPSS was used to analyze the genetic relationship between polymorphisms of H-FABP gene and the association with intramuscular fat content.There were one mutation (A1017G) and one vacancy (G999-) at this gene locus. A1017G makes up a new Haeâ…¡restriction enzyme cutting site, G999- makes up a new Sauâ… restriction enzyme cutting site. A1017G can be analysised by Haeâ…¡-RFLP, and there were BB, Bb, bb three genotypes. The BB was the predominant genotype. A chi-square analysis suggested that the allele frequencies and genotype frequencies of H-FABP gene were not in Hardy-Weinberg equilibrium in the ChongMing white goats, the ChongMing hybridized goats and the XuHuai goats, and the PIC values of these goats were all low, PIC<0.25; There was only BB genotype in the GuanZhong milchgoats, and there was no polymorphisms. G999- can be analysised by Sau I-RFLP, and there were DD, Dd, dd three genotypes. The DD was the predominant genotype. A chi-square analysis suggested that the allele frequencies and genotype frequencies of H-FABP gene were not in Hardy-Weinberg equilibrium in the ChongMing white goats, and the PIC value was low, PIC<0.25; There was only DD genotype in the ChongMing hybridized goats, the XuHuai goats and the GuanZhong milchgoats, and there were no polymorphisms, the skewness is serious. H-FABP gene increased intramuscular in the order of Bb>bb>BB, dd>Dd>DD. It meant that the fragment of H-FABP had polymorphisms, which could be used as a candidate gene associated gene with phenotypic traits like intramuscular fat content in different goat populations.