The CDNA Sequences And MRNA Expression Pattern Of Duck A-FABP And H-FABP Gene

Fatty acid binding proteins (FABPs) are a group of small molecular weight proteins with high binding affinities for fatty acids and transport fatty acids to sites of fatty acid oxidation, of fatty acid esterification into triacylglycerols or phospholipids. Therefore, FABPs play an important role in the modulation of lipids metabolism. The experiments were designed to clone duck's cDNA sequences of A-FABP and H-FABP gene based on the sequences of poultry and mammal A-FABP and H-FABP gene by comparative genome method; and study the mRNA expression patterns by measuring the different expression levels of A-FABP and H-FABP gene between the control group and the overfeeding group Cherry Valley ducks by RT-PCR; Studying the patterns about the tissue mRNA expression of A-FABP and H-FABP gene in the process of Cherry Valley duck,s growth and development. The main results are as follows:(1) Overfeeding can significantly increase liver weight, percentage of liver, abdominal fat weight and percentage of abdominal fat of Cherry Valley ducks (P<0.05). The concentrations of Total Cholesterol (TC), Triglyeride (TG), High Density Lipoprotein Cholesterol (HDL-C) and Low Density Lipoprotein Cholesterol (LDL-C) in the blood of the overfeeding group were higher than that in the control group, but no significant different was found (P>0.05).(2) The cDNA sequences of duck A-FABP and H-FABP gene were cloned. (GeneBank Accession Number:DQ358123;DQ839646).(3) The mRNA expression levels of duck A-FABP gene in liver, muscle, spleen, lung and ovary of the overfeeding group were higher than that in the control group respectively (P<0.05). In abdominal fat and muscular stomach, the expression levels of A-FABP in the overfeeding group were lower than that in the control group respectively(P<0.05). But no significant difference was found in other tissues between the two groups (P>0.05);(4) In heart, liver, diencephalons, leg muscle, lung and ovary, the mRNA expression levels of H-FABP gene in the overfeeding group were higher than that in the control group respectively (P<0.05). In abdominal fat, the expression levels of H-FABP in the control group was lower than that in the overfeeding group (P<0.05). But, no significant difference was found in other tissues between the two groups (P>0.05).(5) The mRNA expression patterns of duck A-FABP gene in the tissues were different in the process of duck,s growth and development. No mRNA expression of A-FABP gene was found in diencephalon, no mRNA expression level change was found at different periods in liver, and was only found in kidney of the 23 days old embryo. From the 23 days old embryo to the 56 days old ducks, the mRNA expression levels of A-FABP gene decreased gradually in glandular stomach, intestine and lung of ducks (lung of the 23 days old embryo was excluded), but they increased step by step in heart and muscular stomach.(6) The mRNA expression patterns of A-FABP gene in the tissues were different in the process of duck,s growth and development. From the 23 days old embryo to the 56 days old duck,, the mRNA expression of H-FABP gene did not obviously changed in heart and lung (lung of the 23 days old embryo was excluded), and was not found in liver. The mRNA expression level of H-FABP gene was the highest in glandular stomach, intestine, diencephalons and leg muscle of the 23 days old embryo. It decreased gradually in these tissues after birth. The mRNA expression levels of duck H-FABP gene in muscular stomach increased before the 28 days old ducks, but it was lower at the 56 days old than that at 28 days old ducks (P>0.05).(7) The mRNA expression patterns of A-FABP and H-FABP gene were similar in abdominal fat tissue in the process of duck,s growth. The expression levels of A-FABP and H-FABP gene decreased gradually from 14 to 42 days old ducks, and the gene expression level at 56 days old were significantly higher than that of 14, 28, 42 days old ducks (P<0.05).