The Research Of Trichoderma Development And The Esource Utilization Of Culture Medium

Trichoderma is one of the important biocontrol funguses of plant diseases. Themain issue is about researching the TGY-01strain of biological characteristics,screening of optional soild medium for the diphasic fermentation, prepareing thesuitable promoter for powder formulations, collecting the powder, testing the qualityparameters of the powder formulation and studying the medium conditions ofxylanase production by the liquid-state fementation.Firstly, the powder of Trichoderma production process was developed and wasteof edible fungus residue was used for the solid mediums.The edible fungus residue was one of the best solid mediums by using the qualityparameters of the strain spore production pre gram. It was collected strain about2.226billion pre gram after7days.The results illustrated that strain growed quickly and spore production quantityon the surface of the solid fermentation. Thin culture method (medium thickness of0.5cm,1.0cm,2.0cm,3.0cm) and small attaching-stack culture were used. All theresults were similar, which obtained the number of TGY-01strain spore productionbetween2.186billion pre gram and2.357billion pre gram.The thin way for solidfermentation (0.5cm) improved the quality of the unit sporulation and the sporeproduction quantity reached amount to2.357billion pre gram.Secondly, the inhibition of the TGY-01strain on the antagonism of pathogenicfungi were tested.The results showed that the strain had inhibitory effect on Rhizoctonia solani,Sclerotium hydrophilum Sacci and Fusarium oxysporum by using of confrontation,the inhibition rate were100.00%,80.95%,60.00%, respectively. One of the Fusariumoxysporum inhibition was formed a distinct inhibition zone, and Rhizoctonia solaniand Sclerotium hydrophilum Sacci inhibition were covered and invasived intopathogen's colony.Thirdly, biological characteristics of the strain TGY-01Trichoderma werestudied. The biological characteristics of the strain TGY-01Trichoderma were screenedand investigated. The results showed that the glucose was as the best carbon source,NaNO₃was the best nitrogen source. Adding the NaCl to the medium had effect onthe strain spore production, the suitable concentration of NaCl to the medium wasbeneficial to spore production; the optimum concentration of NaCl to promoting thestrain spore production was0.1%. The mineral elements S, K, Mg and P had an effecton the TGY-01strain growth and the strain spore production. The medium of PSAwas suitable for strain growth, and the best pH was4.The result of testing anti-highand cold temperature showed that the conidia of strains in a55℃under saved after10minuets was still germinated.Using the layered method of collection was one of the ways to decreasing wastethe solid medium and the spore production of strain is quite the original sporeproduction after stirring3days.Fourthly, the quality parameters of strain conidia and the development ofpowder.The quality parameters of the TGY-01strain conidia were that the number ofTGY-01strain was more than11billion pre gram with the haemacytometer andmicroscope. The water content of the powder was less than13.50%with oven on100℃for2hours for3times. The germination rate of the powder was more than95.00%on the peptone-sucrose medium at28℃for12hours.The research on biocompatibility and the germination rate of the powderformulation showed that the carrier was kao lin, the wetting agents was Tween-80,and the dispersing agents was PEG6000. The powder formulation of Trichodermapreparation was confirmed as: the content of Twain-800.2g, PEG60000.4g,0.4gpowder, added kao lin to5.0g. Testsed on quality parameters of powder formulationthe showed that the dispersion time was13.57minutes, the water content of thepowder was4.37%, the fineness of the power was92.25%(through No.400narrowsieve), the number of conidia was10billion pre gram.Fifthly, selecting the optimal condition of xylanase production about theTGY-01strain. The influences of fermentation time, the carbon source, the nitrogen source,quantity of Surfactant, concentration of carbon source and inoculation amount of thestain on xylanase production was studied. The result showed that the best carbonsource was glucose, the best nitrogen source was soda nitrate, and the optimalfermentation time was3days, the higher content of carbon source, the higher ofxylanase production, the best content of the surfactant Tween-80was0.15%.