Expression Of Caveolin-1 And Its Correlation With HPV Infection In Laryngeal Squamous Cell Carcinoma

Background:Among these was 50 to100nm inaginations of the plasma membrane referred to as either caveolae intracellulare, due to their cave-like appearance, or plasmalemmal vesicles. The molecular nature of caveolae could be explored following the identification of its signature protein, caveolin. Caveolae being implicated and demonstrated to be important in a variety of cellular functions including endocytic processes,cholesterol and lipid homeostasis, signal transduction,and tumor suppression. Although caveolae and the caveolins are continuously implicated in an increasing array of cellular processes, it is clear that their physiological roles are vastly different depending on the cell type and organ system examined. In this regard, insights into caveolar function will not only be interesting from the stand point of cell biology but will be rewarding in understanding mammalian physiology with applications to human disease. Human Papilloma Virus (HPV) infection is the major etiological factor of Laryngeal squamous cell carcinomas (LSCC). Most of the LSCC patients complicated with HPV 16 and/or 18 type infection. HPV can promote the proliferation and oncogenesis of the basal epithelial cell, but the mechanisms are not clear. One of the mechanisms is that HPV can lead into the basal epithelial cell by Caveolae, so that they lead to the formation of LSCC. The pathogenesis of HPV has been one of the important aspects of the study for etiology of laryngeal carcinoma virus. This paper analyzed the relationship of the Caveolin-1 to the occurrence of laryngeal carcinoma and the relationship of laryngeal carcinoma to the HPV16/18 infection and the relationship between the HPV 16/18 infection and expression of Caveolin-1 to the location, differentiation, metastasis and recurrence of laryngeal carcinoma. It is the purpose of this paper to provide a link between the expression of caveolae in LSCC and the level of HPV and to provide useful information for the predication and treatment of laryngeal carcinoma as early as possible.Objective:To investigate the expression of Caveolin-1 and its correlation with the infection of HPV in human LSCC and to analyse their influences on the biological behavior of human LSCC. This study aim to provided a basic theory and evidence to gene therapy for human LSCC.Methods:Formalin-fixed and paraffin embedded LSCC cases from 66 Laryngeal neoplasms patients and 42 cases with Para-cancerous tissues were tested for Caveolin-1 and HPV 16/18 type expressions by immunocytochemical staining or in-situ hybridization respectively. Results were analyzed with the clinical pathologic parameters and survival.Results:The positive rates of Caveolin-1 in human LSCC and Para-cancerous tissues were respectively 71.2% and 9.52%(p<0.05). Expression rate of Caveolin-1 were significantly associated with tumor differentiation, TNM stage and lymph node metastasis. In 66 cases of laryngeal carcinoma.33 cases showed HPV 16/18 expression. The frequency of HPV 16/18 expression in laryngeal carcinoma and Para-cancerous tissues were 55.1% and 21.4% respectively (p<0.05) and there was no relation with differentiation and lymph node metastasis (p>0.05). The expressions of Caveolin-1 and infection of HPV in LSCC had a positive correlation(r=0.62,χ2 = 10.40, p<0.05).Conclusion:Expression of Caveolin-1 was significantly higher in human LSCC than in Para-cancerous tissues. Caveolin-1 had significant relations to tumor invasion and lymph node metastasis. The pathogenesis of laryngeal squamous cell carcinoma was closely related with the HPV 16/18 infection. And the mechanism might be that Caveoiln-1 mediated the infection of HPV in human LSCC.