| Background and Aims:Immune dysregulation plays an important role in the pathogenesis of ulcerative colitis (UC). IL-8 and IL-10 are the important cytokines of promoting and restraining the immune response for maintaining immune balance. The aims of study were to compare the effects of traditional Chinese medicine Kuikeling and its simplified formula on the serum level of IL-8 and IL-10 in colitis rats model。Methods:A total of 56 Sprague-Dawley rats were randomly divided into four groups which were normal control group (A), Kuikeling-treated group (B), Kuikeling Simplified formula-treated group (C) and colitis model group (D), respectively. Colitis model was induced by TNBS. The rats were killed separately in the acute and remission stages of colitis. The levels of serum interleukin-10 (IL-10) and interleukin-8 (IL-8) were measured by enzyme-linked immunosorbent assay (ELISA). The histological score of colon was also evaluated.Results:Compared with group D, the general conditions and histology of colon in group B and C were slightly improved. But there was no statistical difference between B and C. In the acute stage, the level of interleukin-8 (IL-8) in group B and C was higher and the level of interleukin-10 (IL-10) was lower (P<0.05) than that of group A, but there was no statistical difference in the level of interleukin-8 (IL-8) and IL-10 between group B and C (P>0.05). In the remission stage, the levels of interleukin-8 (IL-8) and interleukin-10 (IL-10) in group B and C were lower and higher than those in group D, respectively (P<0.05), but there was no significant difference between group B and C (P>0.05).Conclusions:The traditional Chinese medicine Kuikeling and its Simplified formula have obvious therapeutic effect on colitis in rats. The mechanism may be related to promoting secretion of anti-inflammatory cytokine IL-10 and inhibiting secretion of cytokine IL-8. |
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