Effect Of Traditional Chinese Medicine Kuikeling On The Expression Of TGF-β1 In TNBS-Colitis Model Rats

Objective:The study was to investigate the effect of Traditional Chinese Medicine Kuikeling on the expression of transforming growth factor-β1 (TGF-) with reparation of mucosa in colitis model rats, and to explore the possible mechanisms of Kuikeling in the treatment of IBD.Method:Sprague-Dawley rats TNBS-Colitis model was induced by trinitrobenzene sulfonic acid (TNBS). The changes of physiological conditions (body weight, food and water intake), histological score of colon and the serum levels of IL-8 and IL-10 were evaluated in the experiment. The expression of transforming growth factor-β1 (TGF-β1) and vascular endothelial growth factor(VEGF) in colonic mucosa were measured by immunohistochemistry(IHC).Results:Kuikeling could obviously improve the general conditions and histological scores of colon in TNBS-colitis model rats.The expression of TGF-β1 in colonic mucosa was obviously increased, but the expression of VEGF in colonic mucosa has no obvious change. In the acute stage, the levels of interleukin-8 (IL-8) and interleukin-10 (IL-10) in colitis model group were increased and decreased respectively. In the remission stage, the levels of plasma interleukin-8 (IL-8) and interleukin-10 (IL-10) were decreased and increased respectively.Conclusion:The results indicate that Kuikeling plays an important role in colonic mucosal reparation in colitis model rats. The mechanism may be due to up-regulating the expression of TGF-β1 in colonic mucosa and regulating the secretion of plasma inflammatory cytokine IL-8 and anti-inflammatory cytokine IL-10.