| Ginsenoside Rh2 which is the trace element of ginseng leaf, red ginseng and American ginseng leaf, has a strong anti-cancer activity, and low toxicity. It has a broad development prospects, as its preparations have used for anti-cancer. Ginsenoside H is an active compound prepared from total ginsenosides in the leaves of panax quinquefalium by alkaline degradation. Ginsenoside H for injection is filled and sterilized after being added polyoxyethylene castor oil esters -35 triglycerides as an excipient. Ginsenoside H is designed to be used for Replenishing qi and blood, Inhibition of tumor growth, and improveing immune function.Through a large number of experiments and literature, the preparation of the test solution of American ginseng leaf is studied. The American ginseng leaf, Ginsenoside H and preparation of Ginsenoside H are also studied systematically using HPLC. Detection standard drafts of the fingerprint of them are drawn up elementally. A single component of Ginsenoside Rh2 is gained and characterized, which will be used as a reference standard testAccording to instructive rules of "Fingerprints of TCM Injections Technical Guide" and "Chromatographic Fingerprint of TCM injections Experimental Technology Guide " from the State Food and Drug Administration and related literatures, we set up the fingerprint chromatogram condition for the analysis of the leaves of Panax Quinquefolium Linn.The analysis was performed on octadecyl silane bonded silica column (column temperature:40℃) with acetonitrile (A)-0.05% phosphoric acid(B, contains 2% tetrahydrofuran) as mobile phase with gradient elution at flow rate of 1.0 mL/min. The UV detection wavelength was 203 nm. The gradient elution was carried out as follows: 0-18 min, A: 20%; 18-58 min, A: 20% to 40% (linear gradient); 58-73 min, A: 40% to 100% (linear gradient); 73 -80min, A: 100%. It was indicated that all the fingerprint chromatograms of the leaves of Panax Quinquefolium Linn had 14 shared peaks and that similarity of chromatograms of leaves of Panax Quinquefolium Linn varied from cultivation areas: chromtogram similarities of those from the Northeast were superior( >0.9); those from Shangdong were also good; but chromtogram similarity between thsoe from the Northeast and those from Shangdong was not so good (0.8-0.9). Thus, it is suggested that leaves of Panax Quinquefolium Linn from same cultivation area should be selected when needed because of better similarity.Ginsenoside H, which is degradated from total ginsenosides, is isolated, purified to obtain Ginsenoside Rh2. The physical and chemical properties of the sample analysis shows that melting point and specific rotation accord with the result reported. The study of analysis of its structure by UV, IR, MS, NMR showes that test data obtained with the reported agreement. Stucture of ginsenoside Rh2 is determined to 20 (S) - ginsenoside Rh2, and which can be used as a reference substance.In contrast Rh2 for quercetin ginseng times respectively, the ginseng times glucoside H and preparation for research, through chromatographic fingerprint condition optimizing, method of stability, precision and repeatability of study to establish the ginseng times mangiferin fingerprint of H and preparation of chromatographic conditions: with C18 alkyl silane bonding silica gel for filling agent (optimization Discovery C18 column chromatography); Acetonitrile - water (including 0.1% of phosphoric acid) as mobile phase, ses gradients: 0 ~15min, 40 ~ 55% acetonitrile/water, 15 ~ 55min, 55 ~ 85% acetonitrile/water, 60min, 55 ~ 85% acetonitrile/water, 203nm detected wavelength, Column temperature 40℃. Built ginseng times glucoside H and preparation of the fingerprint, the experimental results show that this paper establish fingerprint of detection method has good stability and reliability can better reflect intermediates and preparation of active ingredients of correlation... |
PDF Full Text Request