Microbial Transformation Of American Ginseng And Analysis Of Its Products

As a traditional medicine and food homogenous material,Ginseng plants have been highly praised by people for their good pharmacological and health effects.American ginseng,as a ginseng plant,has good effects on anti-cancer,anti-hypertension,improving immunity,analgesia and sexual health care.The main active ingredients of American ginseng are polysaccharides,amino acids,peptides,ginsenosides,etc.Among them,ginsenosides are the most important pharmacologically active ingredients.Ginsenosides are divided into ordinary ginsenosides and rare ginsenosides.The rare ginsenosides have stronger pharmacological effects,but Its content in American ginseng is very small,and some rare saponins such as C-K are not even present therein.The traditional method of chemical preparation of rare ginsenosides has the disadvantages of poor reaction control,environmental pollution,etc.The biotransformation method has the advantages of strong specificity,stable reaction and environmental friendliness,and has become a hot spot in the field of health food research.In this experiment,the medicinal and medicinal fungi used American ginseng powder to ferment,produced rare ginsenosides,combined the pharmacological activity of the food and medicinal fungi with the activity of rare ginsenosides,and obtained fermentation materials with extremely high pharmacological and health care value,which is highly efficient and high for American ginseng and provides reference for American ginseng high added value and efficient utilization.In this experiment,51 kinds of edible fungi were screened for?-glucosidase using plate method and PNPG method.The results showed that strains PHL31,25,60,STHR4,PHB29,BZ7,JG04,C11,YSTRA47,YC01,A total of 15 strains of HEE17,CH01,PIB33,PHI30,and HGZ5 produced good enzymes,which was selected as the biotransformation strain of the root powder of American ginseng.The results of the determination of the polysaccharides of the biotransformation products showed that in the liquid fermentation,the contents of PHI30,HEE17,YC01,CH01,STHR4,and 25 American ginseng powders were greatly increased compared to the control American ginseng powder polysaccharides,and the content of PHI30increased.The maximum was 69.18%.In the solid fermentation,the polysaccharide content of strains HEE17,YC01 and CH01 had a great increase compared with the content of the control American ginseng powder polysaccharide.The content of YC01increased the most,reaching 57%.The total saponin content of the biotransformation product showed that the total saponin content of YC01 increased the most in the liquid fermentation with an increase of 159%.In solid fermentation,the total saponin content of HGZ5 increased the most,with an increase of 240%.Combining the results of the two methods of total saponin fermentation,it shows that the saponin variety and content have undergone great changes during the process of fermentation of American ginseng powder by edible fungi.The results of LC-MS analysis of biotransformation products showed that in the two fermentation methods,rare ginsenosides F1,CK,F2,Rh₂,and Rg₂ can be produced with high yields.The ginsenosides of the same strain in both fermentation methods are also There are some differences.According to the above results,the composition of the four fermented strains with different fermentation time was measured to obtain the maximum fermentation time for the four strains with total saponin content of 20 days,20 days,25 days,and 30 days.The types and contents of monomeric saponins of the fermentation materials of different strains at different fermentation times were obtained,respectively.The fermentation time of the maximum contents of the ginsenosides F1,Rh₂,F2,etc.in the fermented strains of each strain was obtained.