| ObjectiveIn this study, we detected the different stages of serum osteopontin and IL - 18 levels in type 2 diabetic nephropathy patients to discuss their role in the development of diabetic nephropathy and whether there is correlation between the two,in order to provide new und- erstanding of pathogenesis and evolution in type 2 diabetic nephropathy.MethodsWe select 90 patients diagnosed as type 2 diabetes from December 2009 to December 2010 in Tai'an Central Hospital, All the cases were confirmed according to the American Diabetes Association(ADA) 2008 diagnostic criteria. According to urinary albumin excretion rate (UAER) they were divided into 3 groups: a simple group of type 2 DM (DM1 group) 30 patients (UAER <20μg/min). Microalbuminuria group (DM2) were 30: (UAER 20μg-200μg/min).Clinical albuminuria group (DM3) were 30: (UAER> 200μg /min). The normal control group consisted of 30 healthy people,who were no significant difference.with theT2DM group in age and sexuality.Serum level of OPN and IL-18 was detected by means of enzyme-linked immunosorbent assay (ELISA). 24-hour urinary albumin excretion rate (UAER) was measured by radio- immunoassay .Results1.There were no significant differences between normal control group and diabetic nephropathy group in age,waist-hip ratio and body mass index. Compared with normal control group,Diabetic nephropathy group had statis- tically significant differences in systolic blood pressure, diastolic blood pressure,fasting blood glucose,2-hour postprandial blood glucose, HbA1c(P<0.01).2. Serum OPN concentration was significantly higher in diabetic nephropathy patients than normal control(P<0.01). Serum OPN was positively correlated with UAER(P< 0.01),and it had no significant correlation with FBG,2hPG,HbA1c,TG,Scr,BUN.3. Serum IL-18 concentration were significantly higher in diabetic nephropathy patie- nts than normal control(P<0.01). Serum IL-18 was positively correlated with UAER(P<0.01),and it was positively correlated with FBG,2hPG,HbA1c,TG,Scr,BUN.4. Serum OPN and IL-18 had correlation and the correlation coefficient was 0.97. ConclusionThere was an elevation in the serum level of OPN and IL-18 in type 2 diabetic nephropathy patients.The concentration of OPN and IL-18 increased with UAER increase- ing and OPN was positively correlated with IL-18 in type 2 diabetic nephropathy patients. OPN,IL-18 and chronic inflammatory disease may play an role in the development of type 2 diabetic nephropathy. |
PDF Full Text Request