HPV Genotype And Microsatellite Alterations In Tissues Of Condyloma Acuminatum

Objective To explore the possible molecular mechanisms of cancerization in condyloma acuminatum (CA) by detecting the presence of MSI/LOH and the distribution of human papillomavirus (HPV) genotype.Methods 1. Subjects:40 cases of CA,11 cases of cancerized CA were obtained by surgical resection. All cases were proved by histopathology. The peripheral blood samples from the corresponding patients were collected as a control.2. HPV detection: The infection genotype of HPV was also detected by flow-through rapid hybridization technique.3. The detection of microsatellite alterations:Five sites of microsatellite polymorphism of human gene were selected:D3S1234,D9S171,D17S1678,D10S1687和D3 S1611, detecting the presence of MSI and LOH by PCR-denaturing PAGE-silver staining technique.Results 1. HPV detection:The low-risk HPV were the most common genotype in tissues of CA. All genotypes were high-risk HPV in the group of cancerized CA.The infection rate of HR-HPV in the group of cancerized CA was significantly higher than that of the group of CA. There was a significant difference between the two groups(χ2=14.586, P< 0.05).2. The detection of microsatellite alterations:The total incidence of LOH in group of CA and in group of cancerized CA were 22.5%(9/40) and 63.6%(7/11) respectively. There was a significant difference between the two groups(χ2=5.005, P<0.05). D9S171 showed the highest incidence of LOH in the five markers, the second was D17S1678. MSI was not frequent. The incidence of MSI in CA and carcinomatous tissue of CA were 10%(4/40) and 18.2%(2/11). No LOH or MSI was found in the in the normal tissues of foreskin.3. Relationship between microsatellite alterations and high-risk HPV:Based on analysis of the two groups, the accident rate of LOH in high-risk HPV group were higher than those in low-risk HPV group. There was statistical significance (χ2=5.467, P <0.05). All 6 subjects which were categorized as positive MSI were infected with high risk HPV type.Conclusion 1.The incidence of HR-HPV in the group of cancerized CA was 100% which suggests that high-risk HPV may be one of the multiple carcinogenic factors in CA.2. Two microsatellite loci (D9S171 and D17S1678) were sensitive in the investigation of LOH. It shows that the p16 antioncogene and p53 antioncogene may be play an important role in the development of carcinogenesis of CA.3. MSI was not a common phenomenon in the development of carcinogenesis of CA.4. Microsatellite alterations may be relate to the infection of high-risk HPV type.