| ObjectiveThis topic used fluorescence quantitative polymerase chain reaction(FQ-PCR) technology to detect DNA type and load of HPV in patients with condyloma acuminatum,and the patients were followed-up for at least three months,to explore the relationship between viral load and type of condyloma acuminatum with the recurrence,in order to provide a scientific basis for prevention and control of the recurrence of condyloma acuminatum.Methods83 patients are all from the General Hospital of Tianjin Medical University between March 2007 and December 2007.According to norms of the clinic diagnosed as condyloma acuminatum promulgated by the Ministry of Health And Disease Control Department in August 2000.Each patient's clinical data were collected, including gender,age,course of disease,lesions characteristic,primary or relapse, complications and the situation of follow-up.The HPV type and DNA load of 83 cases were detected by fluorescence quantitative polymerase chain reaction (FQ-PCR),and the patients were followed-up for at least three months to observe whether or not relapse.SPSS11.5 software were used for statistical analysis.Result1.Results of 83 CA patients:80 of 83 cases with CA were DNA positive(96.4%),in which 63 cases of simple HPV6/11 type were positive(75.90%),4 cases of simple HPV16/18 type were positive(4.82%),13 cases were HPV6/11 and 16/18 mixed positive(15.66%).2 cases were lost to follow up,81 cases were in line with Conditions.in which 36 cases were recurrence(44.40%).2.The relationship between HPV type and relapse:Of 81 cases with CA,61 cases of simple HPV6/11 type were positive,in which 30 cases were recurrence;4 cases of simple HPV16/18 type were positive and 0 cases were recurrence;13 cases were HPV6/11 and 16/18 mixed positive,6 of which were relapsed.No difference in recurrence was noticed between the types of HPV(P＞0.05).3.The relationship between HPV6/11 DNA load and relapse:74 of 81 cases were HPV6/11 positive(89.16%),of which 32 cases were relapsed;The ranges of HPV6/11 DNA contained in recurrent and non-recurrent group were 3.7×10~4-1.2×10~9 copies/ml and 2.0×10~2～2.4×10~8 copies/ml respectively,the difference between two groups was significant(P＜0.05).4.The relationship between HPV16/18 DNA load and relapse:17 of 81 cases were HPV16/18 positive(20.48%),of which 6 cases were relapsed;The ranges of HPV16/18 DNA contained in recurrent and non-recurrent group were 3.7×10~4～1.2×10~9copies/ml and 2.0×10~2～2.4×10~8 copies/ml respectively,the difference between two groups was not significant(P＞0.05).5.The relationship between CA with other STD infection and recurrence:Of 81 CA cases,there were 30 cases with other STD,of which 18 cases were relapsed(60.0%),51 cases with non-complications,18 cases were relapsed(35.3%). the difference between two groups was significant(P＜0.05).Conclusions1.In this study,FQ-PCR technology were used to detect quantitative of HPV6/11 and HPV 16/18 of 83 patients with CA.Positive rate were as high as 96.4%.2.HPV6/11 is the main type of virus infection in patients with CA in Tianjin. Different types are not correlated with recurrence of CA,but there is a significant correlation between HPV 6/11DNA loads and recurrence of CA.3.Comparison of relapse rates were statistically significant differences between Patients with other STD and non-complications,showing that with other sexually transmitted diseases is a major factor in relapse of CA.4.HPV types and quantitative of 8 cases of pregnant patients with CA were deteced and followed-up,the statistical analysis showed that there was no significant difference between the relapse rate and DNA load when compared with non-pregnant.
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