Study On Traditional Chinese Medicine With Anti-gouty Arthritis Effects

Gouty arthritis is a kind of inflammation caused by deposits of monosodium urate acid (MSU) in the synovia of joint due to continual elevation of serum urate acid in human body. Owning to improvement of human living standard, prevalence of high purine diet leads to a rise of incidence of hyperuricemia and gout in those years, making it an urgent need for drugs that is more excellent in treating gouty arthristis with less adverse effects.In this research, quails were administratered with urate acid suspension (400mg/kg) to induce continual elevation of SUA; Hyperuricemia of mouse was induced by subcutaneous injection of urate acid (400mg/kg), or by gavage of hypoxanthine (500mg/kg) in coordination with subcutaneous injection of uricase inhibtor, i.e. potassium oxonate (400mg/kg). Allopurinol, a xanthine oxidase inhibitor, was found to be able to antagonize both the quails'SUA elevation induced with urate acid and the mouse hypreuricemia induced with hypoxanthine and potassium oxonate, but it was inactive to hyperuricemic mouse induced with urate acid. Benzbromarone, another commenly used drug promoting excretion of uric acid, significantly reduced SUA levels of all the animal models in this research.We established the accurate, robust and reliable method to detect the authentic concentration of serum uric acid with HPLC.Gouty arthritis was induced by injection of MSU into joint of rat' s ankles. Compared with animals injected with saline, animal ankles injected with MSU can be detected swell since time 4h since artificial damage, Volume of the MSU treated ankles reach a peak at 11h, then decrease slowly in the following hours. The process may last for more than 30 hours.Hyperuricemic quails were used to assess TCM-1's potential to decrease SUA concentration of the experimental animals.80% ethanol (V/V) extracts of TCM-1 can significantly reduced SUA concentrations of quails induced with uric acid.Dioscoreae Nipponicae Makino's extracts were found to be able to decrease the level of SUA in hyperuricemia mice induced either by injection of uric acid or by coadministration of hypoxthine and potassium oxonate.Astilbin was found to be able to inhibit swell at rat's ankle due to MSU-induced inflammation, and promote expression of IL-4 in inflammatory joints and differertiation of T helper cells from ThO phase to Th2 phase. Its inhibitory function to swell of inflammatory joints may owe to its ability to down regulate Thl-type cytokines that are pivotal to increase vascular permeability.