Study On Tissue Culture And In Vitro Flowering Of Ligularia Stenocephala

In this paper，using leaves from aseptic plant of Ligularia stenocephalaas explants, the effect offactors which affected the tissue culture, such as plant growth regulators, explants, basic medium at thestage of its callus induction，adventitious buds inductionand rooting culturewas studied，and the effectofacclimatization time and transplantion medium on transplant was also studied. As a result, the bestmediums of every stage were screened out, tissue culture system forL. stenocephala was established.And the carbon sources, plant growth regulators and light application time which affected in vitroflowering were explorated.The main results as following:1Establishment of rapid propagation system in L. stenocephala. The results show that: the suitablemedium in callus induction wasMS+0.05mg·L^-1TDZ+0.5mg·L^-1NAA, and callus induction rate was93.33%.The best leaves in callus induction were unfully expanding leaves, and the tip part of leaves hadbetter in callus induction. Light hastened callus induction of L. stenocephala.The MS medium with1.0mg·L^-16-BA+0.1mg·L^-1NAA was preferable for inducing adventitious shoot.The rate of differentiationwas88.33%.MS+1.0mg·L^-16-BA can be selected to induce adventitious shoot， The rate ofdifferentiation was86.67%.The optimal medium for propagation was1/3MS+0.50mg·L^-1NAA,rooting rate was100.00%,average root number was9.57, and average root length was2.59cm. Besides,1/2MS+0.5mg·L-1NAA can be selected to induce toot.There was obvious promotion to get strongseedling by adding the sucrose concentration to30g·L^-1into medium. The best time of hardeningplantlets was5d, transplanted in the artificial mixture with the ratio of perlite︰vermiculite︰peat （1︰1︰1）, and survival rate was93.33%which the survival rate was the highest.2Exploration the in vitro flowering in L. stenocephala. Carbon sources also played an importantrole on in vitro flowering in L. stenocephala，When the concentration of sucrose was30g·L-1, theflowerbud induction rate was the best. In vitro flowering rate in L. stenocephala was significantdifferencebetween different types and concentrations of cytokinins，and KT1.0mg·L^-1was the best, andflower bud induction ratewas53.33%. In vitro flowering rate in L. stenocephalawas no significantdifference betweendifferent types and concentrations of auxins，and the IAA was better compared with other types. The induction of CCCin vitro floweringin L. stenocephalawas no significant difference. Theappropriate concentration of CCC for inducing flower bud was0.6mg·L^-1.The in vitro flowering waspromoted under the light of10h·d^-1.