Comparision Of Motilin Receptor In Dogs Gastrointestinal Tract Expression

BackgroundMTLR is a G-protein–coupled receptor that previously designatedGPR38.motilin releases in to blood during the interdigestive state, binding the MTLRpromotes propulsion of gut contents along the gastrointestinal tract by provokingphase III of migrating motor complex(MMC); MTLR has also been demonstrated torepresent a clinically useful pharmacological target, so studying the distribution，struction and fuction of the MTLR are potentially valuable for the treatment ofgastrointestinal disorders, such as post-operative ileus, irritable bowel syndrome, andfunctional dyspepsia.ObjectiveTo study the distribution of MTLR in dogs gastrointestinal and compare theexpression strength.Methods1.We used immunohistochemisty(S-P) to detect the expression of MTLR in dogsall gastrointestinal tract2.We detected the expression of MTLR mRNA in dogs all gastrointestinal tractby RT-PCRResults1. We observed that MTLR express in muscle fibers and myenteric plexus ofdogs gastric antrum、duodenum、jejunum、ileum、proximal colon、middlecolon, membrane present sepia, but circular and longitudinal expressingMTLR were no observed, the muscle fiber、myenteric plexus、circular andlongitudinal of terminal colon did not appear to express MTLRimmunoreactivity, staining strength from deep to light wasduodenum→ileum→jejunum→proximal colon→gastric antrum→middlecolon→terminal colon 2. We detected that MTLR mRNA express in gastric antrum、duodenum、jejunum、ileum、proximal colon、middle colon, terminal was not MTLRmRNA expression, the expression of MTLR mRNA from high to low wasduodenum→ileum→jejunum→proximal colon→gastric antrum→middlecolon→terminal colon and same as our immunohistochemisty result.Conclusion1. Immunohistochemisty(S-P) confirmed that MTLR distribution in musclefibers and myenteric plexus of dogs whole gastrointestinal tract expectterminal colon2. Immunohistochemisty(S-P) and PCR confirmed that MTLR express ingastric antrum、duodenum、jejunum、ileum、proximal colon、middle colon,but terminal colon was not MTLR mRNA expression, the expression ofMTLR from high to low was duodenum→ileum→jejunum→proximalcolon→gastric antrum→middle colon→terminal colon.3. Our study suggested that the distribution of MTLR in gastrointestinal tractare different.