Research On Extract Of Water-soluble Protein Of Antler Velvet And The Comprehensive Utilization Of Antler Velvet

Antler velvet in the effective material study has brought wide attention. In thispaper, comparison of different extraction methods of antler velvet water-solubleprotein, choose PEF technology as the object, and optimization of this technology. Atthe same time, choose the optimal extraction method to extract the water-solubleprotein electrophoresis analysis, and the extract of crude protein, the activity of crudegroup points is studied. For comprehensive utilization of velvet antler in thecomposition, this article also continue to pilose antler, acidolysis residue with enzyme,red jujube was developed high calcium drink. In order to improve the extraction yieldeffective substances in antler velvet, deer antler utilization to provide train of thoughtand the theoretical basis, and a test basis. Specific research results are as follows:1. Study on the extraction of antler velvet water-soluble protein and theoptimization of extraction technologyBy single factor test, PEF is chosen as the method of extraction of water solubleprotein in antler velvet, stationary other factors of electric field processing, proteinextraction rate as index, determine the optimal liquid ratio is12, electric fieldintensity is30kV/cm, pulse number is12.Using the ternary quadratic general rotational combination optimization of electricfield processing conditions, mathematical model of the regression equation is asfollows:Y=26.39212-2.53078X₁+2.20700X₂+2.31897X₃-1.60333X₁²-3.04583X₂²-2.83370X₃²+0.26625X₁X₂+0.48625X₁X₃-0.10875X₂X₃Best combination is: the electric field strength is27.03kV/cm, pulse number is12, material liquid ratio is12, extraction rate can reach27.32%at this time.2. The separation of the water-soluble protein in the antler velvet and the studyon the protein’s active movementIdentical samples of protein marker and the water extract of antler velvet wereelectrophoretic analysis by conventional SDS_PAGE, Tricine-SDS-PAGE,SDS_PAGE for polypeptide and the SDS_PAGE containing urea. The separatingeffects of these four methods were compared. Select the SDS_PAGE containing ureaelectrophoresis analysis method for this test method.Compared the ammonium sulfate fractional precipitation, the ethanol precipitation and the ultrafiltration separation, test results show that only the ethanolprecipitation method can avoid to introduce new faults, so choose ethanolprecipitation as the test method.Compared the clearance rate of DPPH and O2-of two kinds of raw ingredients,antler velvet aqueous extract’s small molecular protein and peptide part are strongerin oxidation resistance function, and the function is dependent on concentration.3. Study on the extraction of antler velvet slag water-soluble protein and theoptimization of extraction technologyAccording to different kinds of enzymes PEF applied conditions, and cost factors,choose neutral protease bromelin as PEF in the enzyme solution. Test result provesthat the electric field intensity is15kV/cm and pulse number is8are pineappleproteinase under PEF the optimal enzymatic hydrolysis conditions. Through singlefactor test, choose liquid ratio is8, enzyme concentrations is7U/mg, electric fieldintensity is15kV/cm, pulse count is8.Using four factors quadratic general rotational combination digestion conditionsoptimization mathematical model of the regression equation is as follows:Y=14.32857-0.09458X₁+2.18708X₂-1.79458X₃+0.53792X₄-1.56558X₁²-0.68683X₂²-1.03683X₃²-1.24933X₄²+0.29187X₁X₂+0.08438X1X₃+0.14813X₁X₄+1.08062X₂X₃+0.13187X₂X₄+0.18938X₃X₄Best combination is: the liquid ratio is8, the enzyme concentrations is8U/mg,the electric field strength is15kV/cm and pulse number is8, extraction rate can reach15.96%at this time.After enzyme solution in most of the peptide molecular weight range from25kDa to16kDa. As continuous electrophoresis bands, proved that the molecularweight distribution range is very wide. At the same time, the part of less than16kDacontains some continuous fuzzy electrophoresis bands, proved enzyme solutionproduce part of the small molecule polypeptide.Testing different concentrations of enzymolysis products to the clearance ofDPPH, The results show that the enzymatic hydrolysis product has good oxidationresistance, and under the same concentrations, the Clearance to DPPH is stronger thansmall molecule protein and peptide of aqueous extract.4. Study on red jujube antler velvet high calcium nutrition beverageThe antler velvet residue after the PEF technology acid solution is used to extractcalcium, compound after developed the red jujube juice high calcium drink, throughthe test, determine the sugar acid ratio is50, use60%RA stevia sugar replace17%sugar in the drinks to reduce the beverage calories. Add0.3%calcium lactate andPGA compound stabilizer beverage as a stabilizing agent, the ratio is1:2, this composite stabilizing agent can make the shelf life of drinks more than247days.Through study on the beverage traits, health indicators, determination of physicaland chemical indicators, microbiological indicators, determine the development ofbeverage industry standards. Each200mL beverage contains over800mg calcium,meet the needs of the human body in one day.