Single Nucleotide Polymorphism Of IGF-IR And IGFBP-3Gene Associated With Production Performance In Jinghai Yellow Chicken

In this research, IGF-IR and IGFBP-3gene were selected as candidate genes which affect correlate traits in Jinghai yellow chicken. PCR-SSCP, PCR-RFLP and DNA sequencing were used to detect the SNPs of candidate genes in order to analyse their relationship with production traits in Jinghai yellow chicken. The main results are listed as follows:1. Seven mutations (G26333A, G26336A, C26639T, G110996C, C111014A, C143082G and A143135G) were detected in exons of the IGF-IR gene in Jinghai yellow chicken. Three mutations (T160G、C1087T and C4095T) of the IGF-IR gene were found in Jinghai yellow chicken.2. G26336A and C111014A of the IGF-IR gene lead to Alu I and Hinl I restriction site change.3. Seven mutations of the IGF-IR gene were detected in coding region. Mutations in the coding region of exons were synonymous mutations. Statistic results showed that the locus IGR3-1was devided from Hardy-Weinberg Equilibrium status. The others were in Hardy-Weinberg Equilibrium status in Jinghai yellow chicken.4. Three mutations of the IGFBP-3gene were detected in Jinghai yellow chicken. 1mutation was detected in intron1.1mutation was located in exon2.1mutation was locat ed in exon3. Statistic results showed that the locus IBP2-1was devided fromHard y-Weinberg Equilibrium status. The locus IBP1and IBP3-1were in Hardy-Weinb erg Equilibrium status.5. IGR2-1and IGR16(IGR16-1、IGR16-2) in the exon region of the IGF-IR gene had significant effect on egg number at300days(P<0.05). It suggested that the IGF-IR gene could be the major gene or is linked to the major genes that affect the reproduction traits in Jinghai yellow chicken. For primers IGR2-1, Jinghai yellow chicken with genotype A A and AB had larger egg number at300days than those with genotype BB in Jinghai yellow chicken (P<0.05). IGR2-1had a great potential to be used as genetic marker for egg number at300days in Jinghai yellow chicken.6. IGR2-1, IGR2-2(Alu I), IGR2-3, IGR3-2(Hin1I) and IGR16(IGR16-1、IGR16-2) in the exonic region of the IGF-IR gene had significant effect on growth traits (P<0.05). It suggested that IGF-IR gene could be the major gene or is linked to the major gene that affects the growth traits in Jinghai yellow chicken. For primers IGR2-2(Alu I), Jinghai yellow chicken with genotype DD had larger body weight at8wk of age than those with genotype CC in Jinghai yellow chicken (P<0.05). Jinghai yellow chicken with genotype DD had larger body weight at12wk of age than those with genotype CC and CD(P<0.05). IGR2-2(Alu I) had a great potential to be used as genetic marker for growth traits in Jinghai yellow chicken.7. Linkage disequilibrium analysis showed that IGR16-1and IGR16-2site was in complete linkage disequilibrium. IGR3-2and IGR16-1site was in a strong linkage disequilibrium.8. IBP1and IBP2-1of the IGF-IR gene had significant effect on age at first egg(P<0.05). IBP1of the IGF-IR gene had significant effect on growth traits (P<0.05). For primers IBP1, Jinghai yellow chicken with genotype OP had larger body weight at Hatch weight than those with genotype PP(P<0.05). Jinghai yellow chicken with genotype OP had larger body weight at8wk of age than those with genotype OO(P<0.05). IBP1had a great potential to be used as genetic marker for growth traits in Jinghai yellow chicken.