The Efficient Propagation System Of Xanthoceras Sorbifolia Bunge In Da Qing Area

Xanthoceras sorbifolia Bunge, which belongs to Xanthoceras of Sapindeceae, is a indigenous and rareligneous plant species in North China. In addition to a quite elegant and suitable ornamental tree with greatenvironmental adaptability, it shows more significant value both in energy and medicinal fields.Reproduction of that plant may achieved by means of seeding, cutting, tissue culture, etc. At present, themost prevalent method regarding propagation is seeding reproduction. However, the reproduction ratio isrelatively low and various mutation may exist to certain extent,with such method. Hence,the in-depth study onXanthoceras sorbifolia helps for the development and utilization of the resource of the plant.Through abundant researches on seeding, cutting reproduction and tissue culture of Xanthocerassorbifolia, my experiments seek to find out respective rules for different reproduction ways and to explore themost simple, convenient and high performance reproduction methods, which provide for technical assistancefor large scale cultivation. The results are as follows:1. Seed propagation:The results of different treatments show that,“-20℃refrigeration method” indicates the best performanceamong these methods, mechanic treatment,95%H₂SO₄treatment, different temperature （-20～85℃） treatment,GA₃treatment, moist stratification treatment The “-20℃refrigeration method” toppedwith a germination rateof92.6%, The results also show that refrigeration storage for long time keeping.2.Cutting breeding: Systematic research on the propagation of Xanthoceras sorbifolia cuttings in Daqingarea. The results are as follows:（1）The survival rate with root cutting is obviously higher than that by means of stem cutting forXanthoceras sorbifolia.（2）As for the choosing of cutting sections, it’s better to take those1years parts as cutting slips. The bestquicksets length for cutting is6cm. The best rooting rate, is87.3%.（3） ABT No.1（150ppm）4hours treating shows the best result, with highest rooting rate is87%.（4） The best cutting medium for Xanthoceras sorbifolia is found to be ash: vermiculite: perlite: sand（4:2:2:1）, the root cutting rate is very high.（5） Spring is the best season in Daqing area for cutting according to the result.3. More attention is paid to the researches on cutting medium formula, hormone proportion, inoculationmethod, anti-browning agents transplanting medium, cultivating conditions, late seeding stage management.（1）Seedlings leaves produce both higher survival rate and inductive rate as explants.The best explantsdisinfection, Use75%alcohol surface disinfection for30s at first, and then0.1%mercury dichloride and0.01%Tween as disinfectant solution for10min is best fits for seedlings leaves.（2）The DKW is the best medium for leaf induction and culture. Face-bonding is the best inoculationmethod and the inducting rate is91.4%.（3）The best medium for callus induction of Xanthoceras sorbifolia was DKW+6-BA0.5mg/L+NAA0.5mg/L+2,4-D1.0mg/L the average number of budding callus was93%.（4）The best medium for callus differentiation and proliferation of Xanthoceras sorbifolia is DKW+6-BA0.5mg/L+NAA0.5mg/L+KT1.0mg/L.The best length is1.9cm; the differentiation of callus is70%, and the best increasing coefficient is5.3.（5）The best medium for bud elongation is DKW+6-BA0.2mg/L+IBA0.5mg/L. After20days culture,the average mass growth of bud was grow well.（6）The best medium for rooting culture of Xanthoceras sorbifolia of tissue culture was1/2MS+IBA0.5mg/L.（7）The best anti-browning agent is Vc150mg/L, and the anti-browning rate is17%.（8）The mixture including ash: vermiculite: pearlit（e4:2:3）is the best material, and the survival of plantletscould be up to90%after transplanting soil.