| Cellulose is the main production of plant photosynthesis and the world’s mostabundant renewable energy substances. The crops straw mainly includes cellulose,hemicellulose, and lignin, cellulose and hemicellulose surrounded by lignin. Celluloseresources are extremely rich in our country, Crops straw stalk output up to5.7×108kg a year. But a large amount of cellulose didn’t get to utilize, they always beendiscard to the wild natural decay or been burning. Not only cause the waste of naturalresources and cause pollution of the environment. Because of the cellulose has thehigh crystal structural which have water insolubility,it is very difficult to translate foravailable Glucose．Using the cellulase whichproduced by the microbiology to decompound and translate the cellulose is anefficacious way． At present people on the research of cellulase are mostlyconcentrated on the Mildew, but the most of Mildew is the microbiology which needthe oxygen and the silage fermentation is going on the condition of little or nooxygen，so screening the hign producing cellulase bacteria which the growing courseis not need or little need the oxygen have the big practical.1This investigation isolated84aerobic bacteria which have cellulose-degradingability from ruminal fluid of the fine wool sheep, the cattle and the camels in Xinjiang.40Highly cellulolytic aerobic bacteria were screened out by using Congo red stainingand liquid secondary screening culture mediums. The combination of morphologicaland biochemical test with16SrDNA sequence analysis were carried out for theclassification of the strains. They are including37G-isolates and3G+, Most G-bacteria formed circular bulge，Neat, smooth and moist, translucent pale yellow，opaque yellowish colonies, Some concentric rings, not transparent, cream white,yellow and white opaque colonies in the middle, diameter is1.5-4.0mm, both endsobtuse, some have buds; G+bacteria are mostly formed round or spindle, flat, marginentire, matte white colonies, diameter is2-3mm, some visible buds. Identification ofthese40strains showed that they belong to11species of6genera,16strains inStenotrophomonas maltophilia,10Ochrobactrum,5Sphingobacterium,3Microbacterium,3Paracoccus and2Pseudomonas.2The enzymatic activities of four strains with strong cellulose-decomposing abilitieswere studied under different culture conditions. The results indicated that the strainshave the best performance enzyme producing property when straw powder was chosen as the carbon source; the pH was chosen as5.5－6.0and temperature in37℃condition. In this condition, Y6showed highest FPA enzyme activity and reached10.997U/mL, and the CMC enzyme activity of Nf reached11.149U/mL. When thepaper for the substrate, the FPA enzyme activity and CMC enzyme activity for Nfwere9.979U/mL and5.975U/mL. There are results showed that decomposingabilities of different stains were not the same and the utilization abilities of the samestrain to different sources cellulose were different.3Adding high yield cellulose degradation bacteria Y6and Nf on cotton stalk silage.Van Soest method is applied to determine the cellulose content. The results showedtwo kinds of bacteria can reduce pH value, increased the content of DM and CP, alsodegradate cellulose by produce cellulase. These two bacteria can decreases the neutraldetergent fiber (NDF), acid detergent fiber (ADF) and the content of hemicellulose(HC), and Improved the quality of the cotton stalk silage in different degrees, thecompany of two bacteria have a remarkable effect on improving the quality of silage.4In the process of corn straw silage adding lactic acid bacteria and cellulosedecomposing bacteria at the same time, Make the pH value of corn stover silagedecreased obviously, DM and CP content increased, at the same time make the qualityof NDF and ADF scores dropped by30.5%and19.6%respectively, Hemicellulose(HC) content significantly decreased by50%（P<0.05）. Significantly improved thefermentation quality and nutritional value. |
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