Study On Physiological Mechanisms And Prevetion Measures Of Early Bolting In Angealica Sinensis(Oliv.)Diels

In this study, we conducted research on the physiological mechanisms of early bolting using Angelica growing in the field to be a material based on the regulations of endogenous hormones and protein phosphorylation, and attempted to control bolting or flowering of the Angelica by exogenous treatments. The results are as follows:(1) Polyclonal antibodies with high affinity to GA3-ME and ZR were prepared successfully after we Immunized mice with compounded artificial antigens of GA3and ZR. The indirect ELISA determination systems of GA3-ME and ZR were established, and the endogenous GA3in different status(free、conjugated) were determined as well as ZR during Angelica bolting process. The results show that the contents of GA3in different status and ZR were all increased with the occurrence of bolting, suggesting that increments of GA3and ZR are beneficial to the occurrence of bolting; further more, the contents of GA3and ZR in the same period but different tissues were presented as leaf<leafstalk<root, indicating that root is the principal tissue where GA3and ZR synthesized.(2) Firstly, we used the modified Tris-Phenol method to extract the total proteins of Angelica. Then we used the Fe3+-chelated immobilized metal affinity chromatography (IMAC) column to enrich the phosphorylated proteins of extract from unbolting and bolting Angelica. Finally, we found some differential bands of phosphorylated proteins between unbolting and bolting Angelica plants by Western blot using anti-serine phosphorylated protein antibody and directly determining using phosphorylated proteins fluorescent dye Pro-Q Diamond, indicating that certain regulations does exist in the processes of bolting of Angelica.(3) We lowered bolting rate of Angelica via treating the plant with biosynthesis inhibitors of GA3:Cycocel (CCC) and Paclobutrazol (PP333),but cannot inhibit or delay flowering of Angelica.