The Expression Of Pattern And Correlation Study Of Mononuclear-Myeloid Derived Suppressor Cells In Peripheral Blood Of Gastric Cancer Patients

ObjectivesThis experiment through Flow cytometry to detect the preoperative and postoperativepatients with gastric cancer inhibition of cell in the peripheral blood mononuclear myeloidderived suppressor cells level change, to explore mononuclear myeloid derived suppressorcells in peripheral blood of patients with gastric cancer expression characteristics anddistribution features, for further studies in this function and its role in the formationprocess of gastric cancer lay a theoretical foundation，and to provide a theoretical basis andexperimental foundation for early diagnosis, prognosis and future myeloid derivedsuppressor cells target gene therapy.Methods1. The peripheral blood specimens of97gastric cancer patients and30healthy adultswere collected in the Department of General Surgery of the First Affiliated of SoochowUniversity. Make the expression pattern of peripheral blood Mononuclear cells (PBMC) inHLA-DR-CD14+as monocyte derived MDSCs. Flow cytometry was used to detect andanalyze expression the of MDSCs in peripheral blood mononuclear cells of two groups.2. Analyse the relationship between the biochemical indicators of patients with gastriccancer patients and the proportion of HLA-DR-CD14+MDSCs.3.Use statistical software to analysis the influence of HLA-DR-CD14+MDSCs in tumorprogression and prognosis, combining with clinical data such as age, gender, tumor size,lymph node metastasis, pathological types, degree of differentiation and TNM stages.4. Collect the same in the peripheral blood of30days after surgery in patients withgastric cancer specimens, using flow cytometry instrument detection of MDSCs in theperipheral blood mononuclear cells expression level and correlation analysis and cancer. Results1. The proportion of HLA-DR-CD14+MDSCs in the peripheral blood mononuclear cellsof gastric cancer patients is significantly higher than the healthy humans’(p<0.01).2. The proportion of T3and T4infiltration degree gastric cancer patients is higher thanthe proportion of Monocytes MDSCs of T1and T2infiltration degree patients(p<0.05);gastric cancer patients with lymph-node metastasis express much more MonocytesMDSCs.than not (p<0.05); the proportion of Monocytes MDSCs of III and IV periodgastric cancer patients is significantly higher than the proportion of I and II periodpatients(p<0.05).3. The proportion of Monocytes MDSCs of gastric cancer patients decreasedobviously at the30thday after operation, which is significantly higher than healthyperson(p<0.01).4. The level of peripheral blood Monocytes MDSCs and CRP have positive correlationwhatever preoperative or postoperative.Conclusion1. The proportion of HLA-DR-CD14+MDSCs in peripheral blood of gastric cancerpatients are higher than normal and tumor size, invasion depth, differentiationdegree,lymph node metastasis, TNM stages are closely related and with the gender, age,tumor size, degree of differentiation of no obvious correlation.2. The proportion of Monocytes MDSCs in peripheral blood of gastric cancer patientsdecreases after resection, which suggests that the immune function recovered in a certainextent.3. The proportion of Monocytes MDSCs in peripheral blood of gastric cancer patientswas positively correlated with serum CRP levels.4. MDSCs level can be used in patients with gastric cancer postoperative follow-up andmonitoring tumor recurrence.