Effect And Mechanism Of Rosuvastatin On Expression Of Tissuefactorstimulated By Oxidized Low-densitylipoprotein In Cultured In Vitro Human Monocyte-macrophages Cell

Objective:To investigate the Effect and mechanism of Rosuvastatin on Expression of Tissue Factor in cultured Human Monocyte-macrophages Cell Induced by Oxidized Low Density LipoproteinMethod:Human monocyte cells were isolated by density gradients centrifugation in vitro and incubated to culture4generations for induced to macrophages cells with Phorbol ester (160nmol/ml) induced24h for trial. The human monocyte-macrophages cells were divided into four groups:control group, ox-LDL group, Poly-inosine monophosphate (Poly I) group, rosuvastatin group. The expression of LOX-1mRNA and TFmRNA was assayed by RT-PCR. The enzyme-linked immunosorbent assay (ELISA) were performed to determin the protein content of TF.Results:The Oxidized low-density lipoprotein group compared blank control group with the gene expression of LOX-1mRNA [(3.25156P±0.15772) vs(1±0)]、TFmRNA[(2.522451±0.138967) vs(1±0)] was increased and this was in a concentration-dependent fashion (P<0.01). The Poly-inosine monophosphate group and rosuvastatin group compared with ox-LDL group the expression of LOX-1mRNA[(2.95139±0.157253)vs (3.25156±0.15772)][(2.877343±0.156558)vs(3.25156±0.15772)]、TFmRNA[(1.811956±0.169699) vs (2.522451±0.138967)]、[(1.687701±0.174647) vs (2.522451+0.138967)]were decreased and this was in a concentration-dependent fashion(P<0.05). The ox-LDL group compared with control groupthe expression of TF[(207.7233±1.154701) vs (184.8467±0.871799)]was increased and this was in a concentration-dependent fashion (P<0.01). The Poly-inosine monophosphate group and rosuvastatin group compared with ox-LDL group the expression of TF TF[(197.8733±1.505003) vs (207.7233±1.154701)]、[(202.9567±2.722744) vs (207.7233±1.154701)]were decreased and this was in a concentration-dependent fashion(P<0.05).Conclusion: LOX-1may be Responsible for the expression of TF in Human Monocyte-macrophages Cell induced by ox-LDL, also it can decrease the ox-LDL mediated TF expression. Rosuvastatin is able to down-regulate the expression of LOX-1mRNA in HMMC induced by ox-LDL corresponding with a decreased of TFmRNA and TF expression.