Biological Significance And Expression Of The Cytoskeletal Focal Adhesion Protein Paxillin In Oral Squamous Cell Carcinoma

Backgrond With the development of molecular biology techniques inrecent years, in-depth study of the molecular mechanisms of oral squamous cellcarcinoma becomes hot. The study found that normal oral mucosa develop intooral squamous cell carcinoma after stimulation of a number of reasons，epithelial cells go through a variety of genetics multiple genetic change．Thechange of albumin within cells which composites of cytoskeleton of the cellnetwork plays an important role in the process of oral mucosal lesions．Theinvasion of malignant carcinoma causes cytoskeletal protein reorganization andreconstruction, so that the extracellular matrix has been damaged and cellsmigrate. The paxillin that is determinated in this experiment is a focaladhesion-associated protein. Paxillin is a signal junction protein whosesequence is highly conserved, distributing of focal adhesion and constitutingthe ties of the focal adhesion, conducts signal transduction through theengagement of the extracellular matrix protein and laminin which can causetumor cell to get the ability of migration and invasion.So Paxillin andinvasion and metastasis of tumor cell, there must be some kind of contact..The purpose of this subject is to study the expression of paxillin in oralsquamous cell carcinoma to explore the role of paxillin in oral squamous cellcarcinoma. Immunohistochemical methods detected the pathological specimensthrough surgical resection of186oral squamous cell carcinoma of hospitalizedpatients from January2005to January2010(These patients did not receivepreoperative radiotherapy and chemotherapy before surgeries)，73cases of Oralsquamous cell carcinoma with neck lymph node metastasis,113patientswithout lymph node metastasisd,53cases of normal squamous epithelium as an experimental control group.All patients with an average age of54.3years.Experiments using immunohistochemical detect of paxillin protein expressionand analyzes statistically according to clinical factor.Results: The expression levels of paxillin in oral squamous cell carcinomawas significantly higher than the normal oral squamous epithelium.（p<0.05）.With lower tumor grade of the paxillin expression increased（p<0.05）. Paxillinprotein expression in lymph node metastasis of oral squamous cell carcinomawas significantly higher than those without lymph node metastasis（p<0.01）.There is no significant correlation between the expression of paxillin andthe patient’s age, sex, tumor growth site.（p>0.05）Conclusion: There is significant correlation between the positiveexpression of paxillin and the pathological grade of oral squamous cellcarcinoma, lymph node metastasis, abnormal expression in oral squamous cellcarcinoma suggests tumor with poor prognosis, reflecting the degree ofmalignancy, helping early diagnosis of the degree of malignancy of oralsquamous cell carcinoma, and can be used as indicators to determine the lymphnode metastasis.