Effect Study Of CCINs-DDP To Chemotherapy Sensitivity On Laryngocarcinoma Cells

Objective To investigate the depression effect and mechanism ofcarbon coated iron nanoparticles-diamminedichloroplatinum (CCINs-DDP) to Hep-2laryngocarcinoma cells.Methods Hep-2laryngocarcinoma cells were revivalled andsubcultured,and were divided into control groups, microparticle groups,medicine groups and microparticle-medicine groups in logarithmic growthphase, pretreating fluid were added into every groups,pretreating fluid wasnormal sodium with in control groups,was dispersing medium of normalsodium and CCINs in microparticle groups, was solution of normal sodiumand DDP in medicine groups and was dispersing medium of normal sodiumand CCINs-DDP in microparticle-medicine groups,hibition ratio of cellgrowth were calculated in24h and48h after cell culture, shape of cell wereobserve at3days after cell culture, RT-PCR were taken to detecting levelof Caspase3mRNA and Survivin mRNA in every group at5days after cellculture, inhibition ratio of cell growth, shape of cell,expressive level ofCaspase3mRNA and Survivin mRNA were contrasted along every group.Results At24hour after cell culture in pretreating fluid, growth inhibition ratio of laryngocarcinoma cells were4.1%in control groups,was4.7%in microparticle groups,was19.6%in medicine groupsand was25.6%in microparticle-medicine groups,growth inhibition ratio oflaryngocarcinoma cells was higher in medicine groups andmicroparticle-medicine groups than in control groups and microparticlegroups, growth inhibition ratio of laryngocarcinoma cells was higher inmicroparticle-medicine groups than in medicine groups, growth inhibitionratio of laryngocarcinoma cells was identical between control groups andmicroparticle groups,at48h after cell culture in pretreating fluid, growthinhibition ratio of laryngocarcinoma cells were4.0%in control groups,was4.5%in microparticle groups,was37.2%in medicine groupsand was51.4%in microparticle-medicine groups,growth inhibition ratio oflaryngocarcinoma cells was higher in medicine groups andmicroparticle-medicine groups than in control groups and microparticlegroups, growth inhibition ratio of laryngocarcinoma cells was higher inmicroparticle-medicine groups than in medicine groups, growth inhibitionratio of laryngocarcinoma cells was identical between control groups andmicroparticle groups.At3days after cell culture,cells growth weredepressed in medicine groups and microparticle-medicine groups,cellgrowth was torpid,adherence was decreasing, apoptosis was occurred,thechange was more notable in microparticle-medicine groups. At5days aftercell culture, RT-PCR were taken to detect expression of Caspase3mRNA and Survivin mRNA in every groups, expressive level of Caspase3mRNAwas0.84±0.14in control groups,was0.87±0.16in microparticle groups,was1.41±0.25in medicine groupsand was1.93±0.27in microparticle-medicinegroups,expressive level of Caspase3mRNA was higher in medicine groupsand microparticle-medicine groups than in control groups and microparticlegroups, was higher in microparticle-medicine groups than in medicinegroups, was identical between control groups and microparticle groups,expressive level of Survivin mRNA was1.34±0.17in control groups,was1.29±0.13in microparticle groups,was0.84±0.19in medicine groupsandwas0.52±0.14in microparticle-medicine groups,expressive level ofSurvivin mRNA was lower in medicine groups and microparticle-medicinegroups than in control groups and microparticle groups, was lower inmicroparticle-medicine groups than in medicine groups, was identicalbetween control groups and microparticle groups.Conclusions CCINs-DDP could increase the expression ofCaspase3mRNA and decrease expression of Survivin mRNA in Hep-2laryngocarcinoma cells,increase of growth inhibition ratio of Hep-2laryngocarcinoma cells and improve chemotherapy sensitivity of Hep-2laryngocarcinoma cells to DDP,improve therapeutic efficacy ofchemotherapeutics.