Studies On Protective Effect Of Trichosanthes Kirilowii Maxim And Allium Macrostemon Bge On Myocardial Ischemia-reperfusion Injury In Rat

Objective:To investigate the protective effect of Trichosanthes Kirilowii Maxim and Allium macrostemon Bge (TKAM) on myocardial ischemia reperfusion injury (MIRI) in rat, and investigate its mechanism initially.Method:The MIRI model was made by occluding the anterior descending of the left artery (LAD) in rat. After30min, undid the slipknot, and kept restoring blood flow perfusion for60min. Set TKAM compound group, single TK group, and single AM group, a negative control and positive control.(1)The standard ECG leads II segment of ST potential values for indicators was observed in rat with myocardial ischemia electrocardiogram effect.(2) Detection of serum creatine kinase isoenzyme (CK-MB) activity and lactate dehydrogenase (LDH) activity, and the level of serum malondialdehyde (MDA), superoxide dismutase (SOD) levels in MIRI model rat.(3)Using Evans blue and TTC double staining method in identifying ischemic risk area and infarct size.(4) On myocardial tissue were examined by HE staining observation of pathological changes of myocardial necrosis, and by electron microscopic observation of the rat myocardial ultrastructural changes.(5) Determination p38MAPK expression by Western Blot. Results:(1)The TKAM group against myocardial at the stage of ischemia and reperfusion phases of ST segment elevation, compared with the model group had significant differences (P<0.01).(2)Compared with the sham group, the serum CK-MB, LDH activity increased, MDA decreased, the activity of serum SOD significantly decreased in model group (P<0.05). Compared with the model group, TKAM group can significantly decrease the activity of serum CK-MB and LDH, and decrease the content of MDA, increase the activity of SOD, there were significant differences (P<0.05or P<0.01).(3)Compared with the model group, except the sham group, ischemia area (RA/LV) in each group on the role of no difference. Compared with the model group, TKAM group can significantly reduce infarct size (P<0.01), There are differences between single herb group and TKAM group, TK group with TKAM group have significantly differences.(P<0.01); AM group with TKAM compared differences (P<0.05).(4)Light microscopic and electron microscopic observation, TKAM could significantly reduce myocardial interstitial edema, myocardial fiber fragmentation degree, and improve the fibrils arranged in a disordered condition, reduce the swelling of mitochondria and intercalated discs distortion degree, make the myofilament neatly arranged.(5)TKAM can obviously inhibit p38MAPK phosphorylation in the ischemia reperfusion myocardium.Conclusion:TKAM has obvious protective effect on rat myocardial ischemia reperfusion injury. It can improve the performance of ST segment of electrocardiogram, and significantly reduce the levels of serum CK-MB, LDH activity and the content of MDA, increase the activity of SOD. TKAM can also obviously reduce myocardial infarction area, reduce the myocardial interstitial edema, and improve fibrils arranged in a disordered condition, reduce the swelling of mitochondria and intercalated discs distortion degree, make the myofilament neatly arranged. Mechanism of protective effect may be related to the inhibition of p38MAPK phosphorylation.