Protective Effect And Mechanism Of Cordyceps Artificial Compound On Renal Tissue In Non-heart-beating Donors Rats

Objective: To investigate the effect and mechanism of cordyceps artificial compoundon renal tissue in Non-heart-beating donor rats. Method: To establish the NHBDs modelwith rat, Divided research animal into three groups randomLy, Cordyceps artificialcompound (CS group n=30), Hypertonic citrate adenine solution (HC-A group n=30),Saline (Nacl group n=18). To observe the renal injury after6h、12h、24h preservation invitro, then detect different renal tubular epithelial cell Apoptotic index (AI), kidney tissueSOD activity and MDA content, Bcl-2/Bax and Caspase-3protein expression. Results:1)After6hours, compared with HC-A group, renal tissue damage was mild in CS group. AIof CS Group was lower (9.72±1.63vs19.50±1.95；P＜0.05, P＜0.05). After12hours,compared with HC-A group, renal tissue damage was mild in CS group. AI of CS Groupwas lower(14.04±1.14vs24.25±2.79；P＜0.05, P＜0.05).After24hours, there was nosignificant difference in three groups (P=0.07).2) After6and12hours, compared withHC-A group, renal tissue SOD of CS Group was higher (148.88±11.69vs91.27±7.65;113.18±8.74vs76.10±4.98), with a significant difference (F=165.47P=0.001;F=179.04P=0.001). After6and12hours, compared with HC-A group, MDA contend ofCS Group was lower (2.73±0.45vs4.66±0.40;3.27±0.35vs5.50±0.36), with asignificant difference (F=142.41P=0.001; F=356.38P=0.001).3) After6and12hours,compared with HC-A group, the expression of Bcl-2/Bax in CS group was significantlyincreased (3.69±0.64vs2.15±0.18；2.08±0.26vs1.28±0.16),(F=72.40P=0.001；F=87.80P=0.001); The expression of Caspase-3in CS group was significantly decreased(10167.96±2150.19vs16499.65±2154.3;14307.06±1406.73vs22653.98±1033.68),(F=60.94P=0.001；F=82.81P=0.001). Conclusions: Cordydeps artificial compound has a better preserve effect on non-hearting beating donor within12hours,Compared withHC-A solution. Which mechanism may be related to the effect of reduce lipid preoxidationand reduce the apoptosis of cell.