Experimental Study Of Huazhuojiedufang In Treating Bile-reflux Gastritis

Objective: To make the rats model of Bile-Reflux Gastritis of Turbid-Toxintype. Observe the effect of Huazhuojiedufang on gastritis mucosa tissue freeradicals and proinflammatory cytokines in rats model of Bile-Reflux Gastritisof Turbid-Toxin type. Study on the mode of action of Huazhuojiedufang inTreating Bile-Reflux Gastritis of Turbid-Toxin type, to provide experimentaldate for clinical research of turbid toxin theory.Method:Part1:The rats’gastritis mucosa tissue morphologic changes of Bile-RefluxGastritis of Turbid-Toxin type.30male Wistar rats were random divided into control group,damp-heatgroup and turbid toxin group,all groups were fed adaptability7days before theexperiment began. The control group routine feed. Damp-heat group wereperfuse with bile15ml/kg into stomach, then put them in the artificial weatherbox of temperature(38±2)℃and humidity(93±2)％, and feed dejecta,lard,peanut oil and feed with scale1:1：1：100for20d. For turbid toxin group, themethods of damp-heat group were taken and with additional5％ethanolsolution and0．5％chili, and were extended the time of establish model and15more days than damp-heat group. Each group was stopped given food butwater for24hs after administration for last time, then cut the belly open toobtain stomach by aseptic technique, then observe the pathomorphology ofgastric.Part2: The effect of determinate the level of SOD and MDA of gastricmembrane and TNF-αin blood serum on the mode of action ofHuazhuojiedufang in Treating Bile-Reflux Gastritis of Turbid-Toxin type.60rats were fed adaptability7days then to take10rats as normal grouprandomly. The other50rats were random divided into model group (1.5ml physical saline gastric perfusion),motilium control group (8mg/kg gastricperfusion), high-dose group of Huazhuojiedufang(27.2mg/kg gastricperfusion),medium-dose group of Huazhuojiedufang(13.6mg/kg gastricperfusion), low-dose group of Huazhuojiedufang (6.8mg/kg gastric perfusion).Besides normal group, the other groups were fed by bile15ml/kg gastricperfusion. Then put them in the artificial weather box of temperature(38±2)℃and humidity(93±2)％, and feed dejecta,lard, peanut oil and feed with scale1:1：1：100, and5％ethanol solution and0．5％chili for35d. At the same timemotilium control group, high-dose group of Huazhuojiedufang, medium-dosegroup of Huazhuojiedufang and low-dose group of Huazhuojiedufang werefed medicine at half an hour’s after creation of models from2th week to5thweek, and control group were fed with1.5ml physical saline. Each group wasstopped given food but water for24hs after administration for last time, thencut the belly open to obtain stomach by aseptic technique, then observe thelevel of SOD and MDA of gastric membrane and TNF-αin blood serum.Results：Part1: The rats’gastritis mucosa tissue morphologic changes of Bile-RefluxGastritis of Turbid-Toxin type.Comparing normal group, infiltration of inflammatory cell of damp-heat group increased obviously, there was significant difference(P<0.01);Comparing normal group, infiltration of inflammatory cell of turbid toxin group increased obviously, there was significant difference(P<0.01);Comparing damp-heat group, infiltration of inflammatory cell of turbid toxin group increased obviously, there was significant difference(P<0.01)Part2:The effect of determinate the level of SOD and MDA of gastricmembrane and TNF-αin blood serum on the mode of action ofHuazhuojiedufang in Treating Bile-Reflux Gastritis of Turbid-Toxin type.Comparing normal group, MDA of gastric membrane and TNF-αin bloodserum of control group increased obviously, there was significantdifference(P<0.01); Comparing model group and motilium control group,MDA of gastric membrane and TNF-αin blood serum of high-dose, medium-dose and low-dose group of Huazhuojiedufang decline notably, therewas significant difference(P<0.01); Comparing normal group, SOD of gastricmembrane of model group decline notably, there was significantdifference(P<0.01); Comparing model group and motilium control group,SOD of gastric membrane of high-dose, medium-dose and low-dose group ofHuazhuojiedufang increased obviously, there was significantdifference(P<0.01).Conclusion：Part1: The rats’gastritis mucosa tissue morphologic changes of Bile-RefluxGastritis of Turbid-Toxin typeThe infiltration of inflammatory cell of the rat Bile-Reflux Gastritis ofTurbid-Toxin type of normal and damp-heat group increased obviously. Theresults show that Turbid toxin can be transformed from damp-heat. Theexperiment made the model of the rat Bile-Reflux Gastritis of Turbid-Toxintype successfully.Part2: The effect of determinate the level of SOD and MDA of gastricmembrane and TNF-αin blood serum on the mode of action ofHuazhuojiedufang in Treating Bile-Reflux Gastritis of Turbid-Toxin type.Huazhuojiedufang could enhance the level of SOD of gastric membrane andremarkably reduced the level of SOD of gastric membrane and TNF-αin bloodserum. So as to reduce oxidative damage and injury of gastric mucosa causedby Bile-Reflux Gastritis of Turbid-Toxin type.