Expression Of Livin And FHIT In Cervical Carcinoma And Their Clinical Significance

OBJECTIVE: We investigated the expression of Livin and FHIT in normal cervicalepithelium, cervical intraepithelial neoplasia and cervical carcinoma. It was aimed tostudy the relationship between Livin and FHIT genes expression of the biologicalbehavior of cervical carcinoma.METHOD: The expression of Livin and FHIT in12cases of normal cervicalepithelium,12cases of cervical intraepithelial neoplasia and56cases of cervicalcarcinoma were detected with immunohistochemistry SP method. Analyzed theexpression of Livin and FHIT, compared with the clinical stage, pathological grade andlymph node metastasis of cervical carcinoma patients.RESULT:1. Expression of Livin and its correlation with clinicopathological factors of cervicalcarcinoma.(1) The positive rates of Livin in normal cervical epithelium, cervical intraepithelialneoplasia and cervical carcinoma were0%(0/12),25%(3/12) and64.3%(36/56)respectively. The differences were significant between normal cervical epitheliumand cervical carcinoma, normal cervical epithelium and cervical intraepithelialneoplasia(P<0.01, P<0.05), The differences was no significant between normalcervical epithelium and cervical intraepithelial neoplasia.(P>0.05)(2) The positive rate of Livin in clinical stage IIa(79.31%) was significantly higherthan the stage I(48.15%)(P<0.05); The Livin aberrant rate in pathological grade III(95%)was significantly higher than the grade II(60%) and grade I (18.2%)cases(P<0.01, P<0.05); In addition, the Livin abnormal rate in cases with lymph nodemetastasis(78.4%) was significantly higher than those without lymph node metastasis(36.8%)(P<0.01).(3) The positive rate expression of Livin in cervical carcinoma had no relationshipwith histologic types、cancerous focus size and patients’age..(P>0.05, P>0.05,P>0.05).2. Expression of FHIT and its correlation with clinicopathological factors ofcervical carcinoma.(1) The positive rates of FHIT in normal cervical epithelium, cervicalintraepithelial neoplasia and cervical carcinoma were91.7%(11/12),75%(9/12) and41.4%(23/56) respectively. The difference was significant between normal cervicalepithelium and cervical carcinma; cervical intraepithelial neoplasia and cervicalcarcinoma (P<0.01, P<0.05). The differences was no significant between normalcervical epithelium and cervical intraepithelial neoplasia.(P>0.05).(2) The positive rate of FHIT in clinical stage IIa (27.6%)was significantly lower thanthe stage I (55.6%)(P<0.05); The FHIT positive rate in pathological grade III(10%)was significantly lower than the grade II(44%) and grade I(90.9%) cases (P<0.05,P<0.01). In addition, the FHIT positive rate in cases with lymph nodemetastasis(21.6%) was significantly lower than those without lymph nodemetastasis(78.9%)(P<0.05).(3) The positive rate of FHIT in cervical carcinoma had no relationship with,histologic types、cancerous focus size and patients’ age.(P>0.05, P>0.05, P>0.05).3.Relationship between expressions of Livin and FHITIn cervical carcinoma The expression of Livin a negative correlation with theexpression of FHIT. The higher the expression of Livin, the lower the positiveexpression of FHIT.(r=-0.438, P<0.01).CONCLUSION: The higher expression level of Livin and lower expression of FHITare involved in the cervical carcinoma cell proliferation, maligant transformation,invasivision and metastasis. Livin and FHIT may both play a important role during theoccurrence and development of human cervical carcinoma.