The Effect Of C-6Ceramide To The Apoptosis And Caspase-3Activity Of Peripheral Blood T Lymphocyte

ObjectiveTo investigate the effect of C-6ceramide to the apoptosis and important apoptosis execution protein Caspase-3activity of peripheral blood T lymphocyte,which has been activated by ConA â…£,in vitro, as the concentration increased. And further to provide the basis test of the feasibility of C-6ceramide,which may induce the apoptosis obstacled T lymphocytes in oral lichen planus to apoptosis.MethodsRandomly selected25healthy people, the selected objects are20-30years old healthy people and no medication history, each extraction15ML peripheral venous blood. Separate the venous blood by lymphocyte isolation liquid and nylon wool column,obtaining about85%purity T lymphocytes. conA (10ug/ml) activating, establish the blank control group, and the different concentration of C-6ceramide:0(A group),5(B group),10(C group),20(D group) umol/l as experimental group. Using immunocytochemical method, get the cell apoptosis rate under fluorescence microscopy,.Using Enzyme linked immunosorbent assay method,study the activity changes of Caspase-3by microplate reader Results1The apoptosis of peripheral blood T lymphocyte in conA+C-6BCD group was much higher than in conA+C-6A group,and as increasing of the concentration of C-6ceramide,the apoptosis of T lymphocyte was also increased. the differences were of statistical significance (P<0.05)2The Caspase-3activity of T lymphocytes in peripheral blood in conA+C-6BCD group was much higher than in conA+C-6A group. and as increasing of the concentration of C-6ceramide the Caspase-3activity of T lymphocyte was also increased. the differences were of statistical significance,(P<0.05)3The apoptosis of peripheral blood T lymphocyte in conA+C-6A group was much lower than in the blank control group, the differences were of statistical significance (P<0.05)4The Caspase-3activity of T lymphocytes in peripheral blood between conA+C-6A group and the blank control group was no significant difference (P>0.05)Conclusions1Activated by ConA, C6ceramide can induce T lymphocyte in peripheral blood of normal human to apoptosis2Activated by ConA, C6ceramide can increase the activity of caspase-3of T lymphocytes in peripheral blood of normal human.