| objective:Dendritic cell (DC) was found in the spleen of the mice, then also found in human peripheral blood.There were many irregular shape bumps like the branches of the tree on its surface for feature. At present, DC is known as the most powerful antigen presenting cell (APC) and can activate CD4+T cell, participate the adjustment of immune tolerance or immune response in the body. Immature DC has the strong ability of capturing antigens, turn into mature DC with the intake of antigens and express the surface markers in its own surface, such as CD80, CD86, S-100and so on as the second signal of activating the CD4+T cell. Studies have shown that DC induced CD4+T cells diferentiate into different T helper lymphocyte (Th) and has a close relationship with the pathogenesis of the airway inflammatory disease such as allergic rhinitis (AR), bronchus asthma (AS) and nasal polyps. In this experiment, the expression level of the CD80, CD86, interferon gamma (IFN-γ) and interleukin4(IL-4) of the nasal mucosa and lung tissue in AR and AS animal models were tested, to kown the expression of DC in the airway inflammation and to understand whether the upper and lower airway inflammatory response has the consistency and to further explore the function and mechanism of DC in the consistency of upper and lower airway inflammation. Methods:In the early experiment, WJ Liu et al chose forty SD rats which were6to8weeks old and divided these rats into four groups according to random number table method. Thses groups included AR group, AS group, control group of AR and control group of AS, each group had10rats. The AR group and AS group were given ovalbumin (OVA), complementary with aluminum hydroxide to stimulate into AS and AR rat model. The control groups were relatively given normal saline and aluminum hydroxide with the same amount. The nasal mucosa and lung tissue of this expriment came from Liu’s animal model. These rats were also divided into four groups which were AR group, AS group, control group of AR (AR con group) and control group of AS (AS con group). Each wax stone was cut into5μm thick pieces and the expression lecel of CD80、CD86、IL-4、IFN-γ in the nasal mucosa and lung tissue were detected by immunohistochemical stainings (SABC method).By counting the number of positive cells, the expression of CD80, CD86, IFN-y and IL-4in the nasal mucosa and lung tissue were observed with the microscope. The relationship between DC and the upper and lower respiratory tract inflammation was also analysed. SPSS16.0software was used to analyze these data. Results:1. The numbers of CD80positive cells in nasal mucosa and lung tissue of AR group were (23.94±4.90) and (25.62±4.02),(2.24±0.45) and (2.38±0.58) in the control groups, respectively. The difference was significant (t values were13.943,18.075respectively, all P<0.05). The numbers of CD80positive cells in nasal mucosa and lung tissue of AS group were (21.90±3.05) and (25.02±3.48),(2.16±0.87) and (2.54±0.86) in the control groups, respectively. The difference was significant (t values were19.661,19.846respectively, all P<0.05).2. The numbers of CD86positive cells in nasal mucosa and lung tissue of AR group were (24.42±4.67) and (22.94±4.34),(2.06±0.53) and (2.04±0.73) in the control groups, respectively. The difference was significant (t values were15.049,15.004respectively, all P<0.05). The numbers of CD86positive cells in nasal mucosa and lung tissue of AS group were (22.42±3.70) and (25.10±4.58),(1.90±0.54) and (2.14±0.35) in the control groups, respectively. The difference was significant (t values were17.377,15.808respectively, all P<0.05).3. The numbers of IL-4positive cells in nasal mucosa and lung tissue of AR group were (141.14±10.73) and (138.46±12.31),(34.96±4.80) and (36.86±5.18) in the control groups, respectively. The difference was significant (t values were28.570,24.065respectively, all P<0.05). The numbers of IL-4positive cells in nasal mucosa and lung tissue of AS group were (139.60±10.50) and (139.24±10.45),(34.38±5.13) and (34.10±4.73) in the control groups respectively. The difference was significant (t values were28.468,28.980respectively, all P<0.05).4. The numbers of IFN-y positive cells in nasal mucosa and lung tissue of AR group were (2.96±0.34) and (2.78±0.33),(32.62±4.33) and (35.46±5.59) in the control groups, respectively. The difference was significant (t values were21.585,18.440respectively, all P<0.05). The numbers of IFN-y positive cells in nasal mucosa and lung tissue of AS group were (2.58±0.52) and (2.66±0.42),(35.24±4.02) and (37.54±4.33) in the control groups, respectively. The difference was significant (t values were 25.474、25.354respectively, all P<0.05).5. There was a correlation between the expression of CD80in the nasal mucosa and lung tissue of AR group (r value was0.729, P value was0.022), same result in AS group (r value was0.639, P value was0.047). There was a correlation between the expression of CD86in the nasal mucosa and lung tissue of AR group (r value was0.870, Pvalue was0.001), same result in AS group.(r value was0.795, P value was0.006).6. There was a correlation between the expression of CD80and IL-4in the nasal mucosa and lung tissue of AR group.(r values were0.755and0.840respectively, P values were0.012and0.002respectively), same result in AS group.(r values were0.849and0.859respectively, P values were0.002and0.001respectively). There was a correlation between the expression of CD86and IL-4in the nasal mucosa and lung tissue of AR group.(r values were0.887and0.673respectively, P values were0.001and0.033respectively), same result in AS group.(r values were0.652and0.729respectively, P values were0.041and0.017respectively).7. There was a correlation between the expression of CD80and IFN-y in the nasal mucosa and lung tissue of AR group.(r values were-0.832and-0.893respectively, P values were0.003and0.001respectively), same result in AS group.(r values were-0.765and-0.734respectively, P values were0.010and0.016respectively). There was a correlation between the expression of CD86and IFN-y in the nasal mucosa and lung tissue of AR group.(r values were-0.804and-0.656respectively, P values were0.005and0.039respectively), same result in AS group.(r values were-0.816and-0.790respectively, P values were0.004and0.007respectively). Conclusions:1.Higher expression of CD80、CD86which were the surface markers of mature DC in the nasal mucosa and lung tissue of AR and AS groups showed that DC took part in the pathogenesis of AR and AS, or the pathogenesis of upper and lower airway inflammation disease.2. As the Th2type cytokines, IL-4was higher expressed in the nasal mucosa and lung tissue of AR and AS, which indicated that IL-4involved in the pathogenesis of AR and AS.3. As the Th1type cytokines, IFN-y was lower expressed in the nasal mucosa and lung tissue of AR and AS, which indicated that IFN-y maybe participated in the pathogenesis of AR and AS.4. There was relevance between the expression of CD80in the upper and lower respiratory tract inflammation. There was also a correlation between the expression of CD86in upper and lower respiratory tract inflammation. At the point of view from the DC, it showed that there was a correlation between upper and lower airway inflammation, meanwhile, DC was related to the consistency between upper and lower airway inflammatory disease.5The expression of CD80, CD86and IL-4(Th2cytokines type) in the nasal mucosa and lung tissue of AR and AS groups was positively associated. There was a negative correlation between the expression of CD80, CD86and IFN-y (Th1cytokines type) in the nasal mucosa and lung tissue of AR and AS groups. It suggested that the mechanism of DC involved in the consistency of upper and lower respiratory tract inflammation was related to DC inducing CD4+T cell into the Th2cells and breaking the balance between Th1and Th2cytokines expression. |
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