Expressions Of CD40、HIF-1a And VEGF In Breast Carcinoma And Biological Effects Of Hypoxia On Breast Cancer M231、MCF-7Cell

Part One Expressions of CD40、HIF-1a and VEGFin breast carcinomaPurpose: To investigate the expression of CD40, HIF-1a and VEGF in ductalcarcinoma in situ and invasive ductal carcinoma and their relation with clinicalpathologic parameter, and analysis the correlation between them.Methods: The expressions of CD40, HIF-1a and VEGF were detected in103casesof invasive ductal carcinoma and14cases of ductal carcinoma in situ withimmunohistochemistry.Results:(1) The positive expression rate of CD40in invasive ductal carcinomawas69.9%, and in ductal carcinoma in situ was21.4%, the positive expression rate ofCD40in invasive ductal carcinoma was higher than in ductal carcinoma in situ (P<0.01).(2) The positive expression rate of CD40, HIF-1a and VEGF was respectively69.9%(72/103),31.1%(32/103) and87.4%(90/103). The expression levels of CD40, HIF-1aand VEGF didn’t correlate with clinical pathologic parameter (P>0.05).(3) Theexpression of HIF-1a was correlated with the expression of VEGF (P<0.001). Theexpression of CD40was correlated with the expression of VEGF (P=0.002). Theexpression of HIF-1a was correlated with the expression of CD40(P<0.001).Conclusions: The expression of CD40in invasive ductal carcinoma was highersignificantly than in ductal carcinoma in situ, and was correlated with the expression of HIF-1a and VEGF, respectively. Thereby CD40provides a target molecule for thebiological treatment on breast carcinoma.Part Two Biological effects of hypoxia on breast cancerM231、MCF-7cellPurpose: To study the effect of hypoxia on human breast cancer M231、MCF-7cells proliferation and the expressions of CD40, HIF-1a and VEGF, and investigate themolecular mechanism.Methods: The growth proliferation of hypoxia on breast cancer M231、MCF-7cells were measured by cck8assay. The mRNA expressions of CD40, HIF-1a andVEGF were assayed by Real-time PCR.Results: Hypoxia significantly promoted the proliferation of human breast cancercells M231in a time-dependent manner (P<0.01, vs normoxia). Similarly, hypoxiapromoted the proliferation of human breast cancer cells MCF-7, and there wassignificant difference compared with the normoxic group (P<0.05, vs normoxia). Theresults of Real-time PCR indicated that the mRNA expressions of CD40, HIF-1a andVEGF were increased in hypoxia-treated M231and MCF-7in a time-dependent manner.Conclusions: Hypoxia can significantly promote proliferation of breast cancerM231、MCF-7cells, and induced the mRNA expression of CD40, HIF-1a and VEGF.