| Objective:To observe the relationship of early fatty liver of laboratory rats withplatelet Active; To observe intervention role of Quzhi Ruangan Decoctionon platelet Active in rats and to explore the mechanism of Quzhi RuanganDecoction therapeutic effect for Early fatty liver.Methods:1.Establishment method: SD male78rats, random into threegroups, the normal group and rats in ordinary feed, model group only27and treatment group and rats with high fat feed (normal feed basis and10%,2%With lard cholesterol production processing factory) to feed.Experimental animals free drink, points cage raised in yunnanuniversity traditional Chinese medicine and animal room, the differencein the experiment, four weekend8weekend,12partial time weekendprofile kill materials, dynamic index observation.2.Group to medicine: built the date of mould, normal group and themodel rats to physiological saline irrigation stomach1ml·200g-1, every day1, to fat liver party according to the observed group soft clinicalequivalent of the conversion, and to go to fat liver herb tea party softfilling stomach1ml·200g-1, once a day.3.Index test: build mode4weeks,8,12weeks, rats blood samples weretaken and the liver tissue from rat liver weight determination, wet weight,calculation of liver weight than the index. Sappanwood fine-Iraq red (HE)dyeing observation liver steatosis situation. Determination of rat liverfunction of serum [alanine amino shift enzyme (ALT), aspartic acidamino shift enzyme (AST)], blood fat [total cholesterol (TC),triglycerides (TG)], liver fat, platelet aggregation rate and blood clotsgrain B2(TXB2),6-ketone-prostaglandin Fl a (6-Keto-PGFl alpha),P-choose meat (CD62p).Results:1. General situation: group compared with normal, model rats, weightgain is more apparent,4weeks model group rats after appear ofdecreased appetite, action activity decline, defecate sticky softaccidentally has rare pond, the color yellow wait for a phenomenon.8weeks model rats, higher than normal weight group and drug group (P <0.05),12weeks model rats, weight higher than normal group and thedrug to continue group, and above four weeks model group and eightweeks model rats, weight (P <0.05).8weeks model group rat liver wetweight than normal group and the treatment group (P <0.05), and the above four weeks model group rat liver wet weight (P <0.05),12weeksmodel rats, liver wet weight increase is more obvious.2. Morphological change: group compared with normal,8weeksmodel group rat liver pathology HE dyed by light microscopy visiblemore than a third of the liver cells appeared in the cytoplasm of varyingsizes circular lipid drops empty bubble.12week model group more thantwo-thirds of liver biopsy visible appeared in the cytoplasm of varyingsizes circular lipid drops empty bubble, the part of the specimen visibleliver cell enlargement, cytoplasm loose reticulation, the cytoplasm ofosteoporosis, or the cytoplasm is almost completely ballooningdegeneration.3.Biochemical indicators: compared with normal group content,4weeks, model rats, elevated serum TG,(P <0.05);12weeks, modelgroup and normal group and the treatment group, compared to increasedserum TG, and higher than8weeks model group (P <0.05).8weeksmodel rats, serum TC content is higher than normal group and thetreatment group (P <0.05),12weeks in model group higher than normalgroup and the treatment group (P <0.05).Rat liver fat content,12weeks model rats, liver TG content is normalgroup and high treatment group (P <0.05);8weekend model rats, liverTC content is normal group and high treatment group (P <0.05),12weeks model group the normal group and high treatment group (P < 0.05).Rats serum transaminase,4weeks model rats, serum AST higher thannormal group and the treatment group (P <0.05).8weekend model rats,serum ALT higher than normal group and the treatment group (P <0.05),and the above four weekend rats ALT content (P <0.05);12weeksmodel rats, serum ALT higher than normal group and the treatment group(P <0.05), and the above four weeks and eight weekend rats ALT content(P <0.05).Rats platelet aggregation rate4weeks model group higher than normalgroup and the treatment group (P <0.05);8weeks model group higherthan normal group and the treatment group (P <0.05), and the above fourweeks model rats, platelet aggregation rate (P <0.05);12weeks modelgroup higher than normal group and the treatment group (P <0.05), andthe above four weeks and eight weeks model rats, platelet aggregationrate (P <0.05).Rats serum blood clots grain B2content4weeks model group thenormal group and high treatment group (P <0.05);8weeks serum bloodclots in rats, B2model group the normal group and the treatment grouphigh model group (P <0.05), and the above four weeks model rats, serumblood clots grain B2content;12weeks serum blood clots in rats, B2modelgroup the normal group and high (P <0.05), and the above four weeksmodel group and eight weeks now rats, serum blood clots grain B2 content.Rats serum6-Keto-PGFl α content4weeks model group the normalgroup and low treatment group (P <0.05);8weeks model group thenormal group and low treatment group (P <0.05), and less than4weeksmodel group (P <0.05);12weeks model group the normal group and lowtreatment group (P <0.05), and less than8weeks model group and fourweeks model group (P <0.05).12weeks treatment group than8weeksand four weeks treatment group (P <0.05).Rats CD62p content in serum8weeks model group the normal groupand high treatment group (P <0.05);12weeks model group the normalgroup and high treatment group (P <0.05), and higher than8weeks andfour weeks model group (P <0.05).12weekend treatment group below8weeks in treatment group (P <0.05).Conclusions:1. Experimental high fat feed of the rats induced by early fatty liverpathological form change in8model weekend formation, performancefor8weekend, model rats, liver HE dyed,10×40, obviously, more thana third of the liver cells appeared in the cytoplasm of varying sizescircular lipid drops empty bubble, some empty bubble fusion will becrowded nuclei to cell membrane.2. Experimental high fat feed of the rats induced by biochemicalindexes in the early fatty liver model4weeks into rising trend, performance for4weeks, model rats, serum transaminase lifts, liverfunction is existence damage, elevated blood fat, liver fat rise.8weekend,12weeks is more obvious. Comprehensive conclusion1show that theexperiment made model success.3.Experimental high fat feed of the rats induced by early fatty livermodel has platelet active, performance for4weeks, model rats, plateletaggregation rate rise, thrombosis, grain B2rise,6-Keto-PGFl α lower, andCD62p rise.8weeks,12weeks is more obvious. That in and platelet ctivelink.4. The use of traditional Chinese medicine Qu Zhi Ruan GanDecoction to be fat liver significantly improve high fat feed of ratsinduced by model for the development of early fatty liver happenedreduce serum and liver total cholesterol, triglyceride obvious effect, canreerse the damage and inhibit platelet activation liver function, preventand cure thereby experimental rat model early fatty liver liver fattychange. |
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