| Objective:To observe the relationship between early fatty liver of laboratory rats andVascular Endothelial Cell, and observe the ill mechanism of early fatty liver fromHepatic Sinusoidal Endothelial Cell damaged. To explore the mechanism of quzhiruangan Decoction therapeutic effect for fatty liverMethods:1.Prepare for the Fatty Liver Rat Model: Separate the eighty-four rats,which areheighted90gram to110gram, into3random groups by the SPSS statistical analysissoftware:24rats for normal group,30rats for model group,30rats for quzhi ruanganDecoction group. All the rats except the normal group were fed with high fatty diet(88％common fodder+10％lard+2％cholesterol). The normal were fed withcommon diet, free drinking water for the experiment in each group. To obtainmaterials for this, all the rats be killed respectively at the weekend of4th,8th and12th.2. Intervention treatment: since the the day of modeling, the rats in normal andmodel group would be fed with saline (1ml/200g/day), quzhi ruangan Decoctiongroup with quzhi ruangan soup (1ml/200g/day), which is made up of pseudo-ginseng,may bloom, chrysanthemum, white atractylodes rhizome and so on, computedaccording to clinical medicine group equivalent amount.3.Detect indicatrix: after modeling for4weeks,8weeks,12weeks respectively,the rats’ blood and livers were taken, the body weight and liver wet weight of ratswere determined, then the ration between liver weight/body weight. the fat degeneration of liver were observed by hematoxylin-eosin (HE) staining; the liverfunction[serum Alanine aminotransferase (ALT), Aspartate transaminase (AST)activity], serum Lipid [Total cholesterol (TC), Triglyceride (TG) content], Livertissue[Total cholesterol (TC), Triglyceride (TG) content], plasma plateletaggregation and Vascular Endothelial Cell (vWF).Results:1. At the weekend of4th and8th, compared with the normal group, althoughthe model rats’ body weight and liver weight increased, the difference has noStatistically significant. The model group and the quzhi ruangan Decoction group alsohave no Statistically significant. At the end of12th, compared with the normal group,model rats’ body weight and liver weight increased（444.23±11.92vs.405.41±47.36，P<0.0001）and（13.17±1.33vs.2.83±1.95，P=0.05）; compared with the model group,the quzhi ruangan Decoction group’s body weight and liver weight decreased（390.33±25.28vs.444.23±11.92，P=0.02）and（11.93±1.37vs.13.17±1.33，P<0.0001）。2. At the weekend of4th and8th, compared with the normal group, liverfunction of the model group has no Statistically significant. At the weekend of12th,the serum ALT of the model group is higher than the normal group（58.00±3.70vs.31.89±1.54, P<0.0001）, the quzhi ruangan Decoction group is lower than the modelgroup （44.38±0.81vs.58.00±3.70, P<0.0001）and the serum ALT of the modelgroup is higher than the normal group （218.50±45.82vs.177.47±21.16, P=0.007），the quzhi ruangan Decoction group is lower than the model group （222.40±8.64vs.239.66±36.03, P=0.025）.3. At the8th weekend, the serum TC of the quzhi ruangan Decoction group islower than the model group（2.61±0.17vs.3.22±0.25, P=0.03）. At the8th weekend,the serum TC of the model group is higher than the normal group（4.83±1.35vs.3.16±0.63, P=0.007）and the serum TC of the quzhi ruangan Decoction group is lowerthan the model group（2.89±0.40vs.3.56±0.58，P=0.015）. At the4th and12thweekend, the serum TG of the model group is higher than the normal group （3.01±1.76vs.1.91±0.78，P=0.05）and （4.83±1.35vs.3.16±0.63, P=0.007）. Atthe12th weekend the serum TG of the quzhi ruangan Decoction group is lower thanthe model group （3.22±0.79vs.4.83±1.35，P=0.015）. At the8th weekend, the liverTC of the model group is higher than the normal group（6.60±0.53vs.5.35±0.38，P=0.006）and Decoction group is lower than the model group（5.86±0.28vs.6.60±0.53，P=0.025）. At the12th weekend, the liver TC of the model group is higherthan the normal group（6.32±0.84vs.5.13±0.73，P=0.015）and the Decoction groupis lower than the model group（5.33±0.86vs.6.32±0.84，P=0.017）. At the8thweekend, the liver TG of the model group is higher than the normal group（3.88±0.82vs.2.68±0.25，P=0.05）. At the12th weekend, the liver TG of the model group ishigher than the normal group（4.07±0.97vs.2.64±0.71，P=0.04）and the Decoctiongroup is lower than the model group（2.99±0.30vs.4.07±0.97，P=0.007）.4. At the12th weekend, the liver vWF of the model group is higher than thenormal （78.14±19.04vs.76.09±5.70，P=0.0001）and the Decoction group is lowerthan the model group（48.08±6.46vs.78.14±19.04，P=0.0001）. At the8th weekend,the platelet aggregation of the model group is higher than the normal （69.42±7.76vs.48.08±6.23，P=0.05）and the Decoction group is lower than the model group（48.47±6.22vs.69.42±7.76，P<0.0001）. At the12th weekend, the plateletaggregation of the model group is higher than the norma（l91.88±6.59vs.77.16±0.87，P=0.01）and the Decoction group is lower than the model group（51.71±5.92vs.91.88±6.59，P<0.0001）.5.By light microscopy，HE staining in different stage displayed that in normalgroup, the organiztion of rats liver is clear and integrity, the shape of Lobuli hepatic isright, the magnitude of liver cells is uniform, round, sticks and central vein can beseen. At the8th weekend, in model group, there were vacuoles of lipid droplets inmore than1/3endochylema of liver cells, some of fusible vacuoles pushed the cellnucleus under the cell membrane. At the12th weekend, in model group, rats liver cellenlarged obviously, it’s yellow, the section of liver cell is greasy, the fat aroundcentral vein pathologically changed, and the volume is bigger than normal, there werevacuoles of lipid droplets in more than2/3endochylema of liver cells, some of fusible vacuoles pushed the cell nucleus under the cell membrane, rats liver cell enlarged, thecytoplasm is loosly, reticularly and translucently. At the8th weekend, in quzhiruangan Decoction group, there were some vacuoles of lipid droplets inendochylema of liver cells, it can be seen in central vein. At the12th weekend, inquzhi ruangan Decoction group, there were some vacuoles of lipid droplets inendochylema of liver cellsConclusions:1. Most pathological changes were found that from the8th week, rats liver cellenlargment, structure illegible, liver cell disorder and there are fat lipid droplets inliver cell. It proved that fedding rats with high fatty diet can made the fatty liver ratmodel in8weeks.2. Vascular Endothelial Cell in the organization of early fatty liver is gettingmuch more. For Vascular Endothelial Cell getting more is one of symbols of HepaticSinusoidal Endothelial Cell damaged, it can be found that there were HepaticSinusoidal Endothelial Cell damage in early fatty liver.3. Quzhi ruangan Decoction can definitely improve pathological changes of theliver function abnormal, blood fat, high lipid and fatty liver changes caused byfedding rats in model group with high fatty diet. Quzhi ruangan Decoction canrestrain Hepatic Sinusoidal Endothelial Cell damage.4. Quzhi ruangan Decoction showed a good intervention in state ofHyperlipidemia fatty liver. It means that restraining Hepatic Sinusoidal EndothelialCell damage and platelet aggregate is one of the principles of preventing fatty liver. |
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