HPV Genotype And Expression Of HMLH1, HMSH2, P16and P53Protein In Bowen Disease

Objective:To investigate the expression and significance of hMLH1, hMSH2, p16and p53protein in tissues of Bowen disease(BD), while explore the role of human papillomavirus(HPVs) in the pathogenesis of Bowen disease.Methods:20cases were proved by pathology in2004-2011, and among them2cases were exposed on crissum and genitals,1case on hand,3cases on other position.10normal group were used as control.21HPV genotypes were detected by human papillomavirus PCR types diagnostic kit in20lesional skins of Bowen disease. Immunohistochemical technique was employed to observe the expression of hMLHl, hMSH2, p16and p53protein in lesional skin in20patients with Bowen disease and in10normal skins.Results:①6patients among the20cases were HPV positive (30%), of which, HPV16, HPV18were detected on lesions of crissum and genitals with the positive rate67%(2/3). HPV16was detected on lesion of hand with the positive rate100%(1/1). HPV16, HPV16,HPV33were detected on lesions of other positions with the positive rate19%(3/16).②The positive rate of hMLH1and hMSH2in BD were lower than that in normal (t’=-6.030,P<0.05)、(t’=-7.153, P<0.05). The positive rate of p16and p53in BD were higher than that in normal (t’=7.225, P<0.05)、(t’=3.647, P<0.05).There was a statistically correlation between hMLH1and hMSH2, p53protein(r=0.553, P<0.05),(r=-0.490, P<0.05).③The expression rates of hMLH1and hMSH2、p16in the infection group were higher than those in the no infection group, and p53were lower than that in the no infection group. Conclusion:①The results of the study support the role of HPV in the pathogenesis of BD in different position.②The abnormal expression of hMLH1, hMSH2, p16and p53protein contribute to the oncogenesis of Bowens disease.③The over-expression of hMLHl, hMSH2protein in HPV-infected lesions of BD might be a kind of feed-back mechanism by HPV. The abnormal expression of p16and p53protein were consistent with the pathogenesis of HPV causing cancer. It’s likely one of the mechanisms of High risk-HPV to lead to BD.