Experimental Comparison Study On Behavior And Ne,5-HT Of That Kaixinsan Herbal Pairs Stressed And Depressed Rats

Objective:Casual observation of Kaixinsan and herbal pairs such as Ginseng-poria cocos group Ginseng-polygala group and Poria cocos-polygala group in chronic unpredictable mild stress and solitary keep depression model rats behaviorand brain monoamine neurotransmitters5-hydroxytryptamine and norepinephrineeffects of serotonin. Explore its mechanism and provide the theory for clinicalmedication.Methods:1. Model preparation: Only60SD clean level male rats,weight200±20g.By weight groups, take picric acid to make points after mark within the groupof rats, according to the group of the whole amount of group simple randomsampling is divided into six groups: the blank control group, the model group,Kaixinsan group Ginseng-poria cocos group Ginseng-polygala group andPoria cocos-polygala group each group of10only. Normal control group every cage only5raised, will not be model preparation. The other5groups with a solitary and chronic mild cannot foresight stress sexual stimulation as a method for depression made rats module, and stimulate and on the time, feed with preventing drugs intervention groups21days. Before the experiment, big ratsadaptability raise3days.2. Feeding on medicine in different groups: On the experiment, give drugdose per day to the herbal pairs according to the Meeh Rubner Fomula. And t hen put it into the normal saline in total2ml. And intragastric administration2ml normal saline per day to the modle group. In the experiment date21formedicine once a day.3. Index detection: Rats gaining weight change, food consumption, sugarwater consumption and Open-field test as behavioral indicators. Ended before the trial and experiment are measured respectively, evolute to Kaixinsan group,Ginseng-poria cocos group, Ginseng-polygala group and Poria cocos-polygala group for chronic stress depression model of behavior change rats. After ending to the experiment of brain5-HT, NE content determination of rats, brain,stripping rapidly rats orgoanization of rats, brain, and separating hippocampus on the ice, after homogenate speed centrifugal, take on the liquid, the enzyme league immune method(ELISA) is the test, and statistical analysis.Results:1. After21days chronic mild cannot foresight stress and solitary raise, compared with normal, each made module rats of behavioral indexes is reducedand the difference was statistically significant(p<0.05or p<0.01), the results show that the model success made.2. With the drug intervention after21days, To compere the model group,rats weights of Kaixinsan group and Ginseng-poria cocos group increase significantly(p<0.05); rats’ food consumption of Kaixinsan group and Ginseng-polygala group increased significantly (p<0.05); rats’behavior activities of Kaixinsan group, Ginseng-poria cocos group and Ginseng-polygala group increased significantly(p<0.05); rats’sugar water partiality of Kaixinsan group, Ginseng-poria cocos group, Ginseng-polygala group and Poria cocos-polygala group enhanced obviously(p<0.05); On the whole, rats’ Brain single amine neurotransmitter5-HT, NE content of Kaixinsan group, Ginseng-polygala group and Poria cocos-polygala group increased significantly(p<0.05), a statistically significant difference. Conclusions:1. A lone with chronic mild unforeseen stress sexual stimulation can be prepared by an ideal depression animal model. The make, model rats and lake ofinterest in the pleasant sensation, activity decrease, explore and depression reduced ability to sample behavior, very good to simulate the clinical depressionof depression in state.2. Kaixinsan group, Ginseng-polygala group can improve significantly the characteristic of CUMS The weights, suger waterpartiality, foot consumptionand vertical and horizontal activity, and so on of two groups have no descended.3. Kaixinsan group, Poria cocos-polygala group, Ginseng-polygala group can increase significantly with brain of monoamine transmitters in5-HT, NE relevant content.4. According to those experiment, in the Kaixinsan group and Ginseng-polygala group infering of CUMS is the most significantly. Second, it’s Ginseng-poria cocos group and Poria cocos-polygala group.5. Difference of groups’ intervension effect provides basic administration and combination for clinical application of curing depressing disease.