Study On The Expression Of Survivin、VEGF、Caspase-3and Their Clinical Significance Of Sacral Chordoma

Part I Relativeness between the expression and the angiogenesis of Survivin and VEGF in Sacral ChordomaIntroduction:Chordoma is a rare and invasive malignant tumor which primarily relies on less effective surgical treatments. In contrast, vascular targeting therapy has a potential to treat malignant tumors. A large number of studies have shown that Survivin and vascular endothelial growth factor (VEGF) play a significant role in the angiogenesis. The purpose of this study is to examine the expression of Survivin and VEGF in sacral chordoma and their association in the angiogenesis.Methods:This retrospective study determined Survivin and VEGF expression immunohistochemcally in30cases of sacral chordoma undergoing surgery in our hospital from January2000to July2010, and analyzed their relationship with clinical and pathological features.Results:The positive expression rates of Survivin and VEGF in the sacral chordoma were70.0%and76.7%, significantly higher than the tumor adjacent tissues (p<0.001and p=0.002). The microvessel density (MVD) in sacral chordoma was27.20±10.70/mm2which was significantly higher than that in the adjacent normal tissues (8.90±3.11/mm2)(p<0.001). The MVD of Survivin-positive in the sacral chordoma was30.71±10.35/mm2, significantly higher than that of the Survivin-negative (19.00±6.25/mm2)(p=0.004). The MVD of VEGF-positive in sacral chordoma was29.43±10.87/mm2, significantly higher than that of the VEGF-negative (19.86±6.18/mm2)(p=0.036). The expression of Survivin and VEGF had a positive correlation (r=0.499, p=0.005). Survivin expression was directly associated with the recurrence (p=0.017),The log-rank the postoperative CDFS showed a significant difference between Survivin positive and negative groups (p=0.005).Conclusion:The expression rates of Survivin and VEGF were high in the sacral chordoma tissues, correlated positively with each other. The vessels of the Survivin-positive or VEGF-positive were richer than those of the Survivin-negative or VEGF-negeative in sacral chordoma. Survivin expression was associated with recurrence.The results of expression and correlation of Survivin and VEGF in sacral chordoma provided a theoretical basis on prognosis of sacral chordoma and targeted therapies. Part II Relativeness between the expression and the apoptosis of Survivin and Caspase-3in Sacral ChordomaObjective:To investigate the expression and the clinical significance of Survivin and Caspase-3in human sacral chordoma tissues.Methods:Expressions of Survivin and Caspase-3were detected in30cases of human sacral chordoma tissues and10normal notochord tissues through streptavidin biotin peroxidase (SP) immunohistochemistry.Results:The positive expression rates of Survivin in chordoma were70.0%,while none of10cases in the normal notochord tissues expressed Survivin, with significant difference between two groups(P<0.05).The positive rate of Survivin was correlated significantly with recurrence (P=0.017);The positive expression rates of Caspase-3was significantly lower in chordoma (33.3%) than that in normal notochord tissues (90.0%),with significant difference between two groups(P=0.003).The positive rate of Caspase-3was correlated significantly with recurrence (P=0.007).Expressions of Survivin and VEGF were correlated negatively with each other (r=-0.463, P=0.010).Conclusion:Survivin expression in sacral chordoma is high, possibly through inhibition of Caspase-3activity, thereby preventing apoptosis and promote tumor cell proliferation. It plays a significant role in cancer development, progression and prognosis.