P53 Expression In The Tissues Of The Esophageal Cancer Patients Serum And Clinical Significance

AIM：To investigate P53level in serum of patients with esophageal squamous cell carcinomabefore and after operation，detect the expression of P53protein in tissues and analyze itsrelationship with clinical stage, pathological features and biological behavior. Provide anobjective basis for the diagnosis of esophageal squamous cell carcinoma, efficacy monitoringand assessment of prognosis.METHODS: Fasting venous blood of55patients with esophageal squamous cell carcinoma inchest Section II of Shanxi Tumor Hospital and30cases of healthy controls should be gatheredfrom July2010to July2011. Automatic ELISA analyzer was used to detect the level of serumP53, while immunohistochemical method was used to detect the tumor tissue and correspondingadjacent normal tissue of P53protein expression.RESULTS:1. The average level of P53in serum of esophageal squamous cell carcinoma patients beforeoperation was1.754±1.161u/ml,0.919±0.761u/ml in normal control group,0.997±0.751u/mlin postoperative control group. The P53levels in serum of esophageal squamous cellcarcinoma patients before operation were significantly higher than normal and postoperativecontrol group, statistically significant difference (P<0.001).2. The preoperative serum P53expression level of esophageal squamous cell carcinoma patientswas no significant difference with age, gender, tumor size, TNM staging and lymph nodemetastasis (P>0.05), but significantly statistic difference with cell differentiation, depth ofinvasion, vascular involved (P <0.05).3. P53positive expression rate was72.7%(40/55) in tumor tissues, no expression in adjacentnormal tissues. There was significantly statistic difference between two groups (P<0.001).4. P53protein expression in tumor tissue was no significant difference with age, gender, andlymph node metastasis (P>0.05), but significantly statistic difference with cell differentiation,TNM staging, vascular involved (P <0.05).CONCLUSION:1.P53was overexpression in the serum and tumor tissue of esophageal squamous cellcarcinoma patients, and related with the occurrence of esophageal squamous cell carcinoma2. Preoperative serum P53expression was significantly higher than normal and postoperativecontrol group, which indicated that the high level of preoperative serum P53was caused by thetumor.P53may be used as the postoperative follow-up monitoring of tumor recurrence andmetastasis of indicators. 3.P53expression level in the serum and tumor tissue correlated with age, gender, and lymphnode metastasis (P>0.05),meanwhile there were significantly statistic difference with celldifferentiation, TNM stage, vascular involving related(P<0.05).4.Esophageal patients serum P53expression and organization P53expression have significantrelationships,can through the serum antibodies to replace the determination of P53organizationP53the determination of protein expression.