Shandong Han Population Folinic Acid (methylene Reductase (mthfr) Gene C677t Gene Polymorphism And The Relationship Between Sudden Deafness

Objective:To learn the prevalence of methylenetetrahydrofolate reductase(MTHFR)C677T genotype and its association with sudden deafness (SD) in Shandong Hans. discuss the occurring mechanism of SD from genetic reason, provide reference for the Causes and prevention of SD.Methods:Polymerase chain reaction—restriction fragment length polymorphism (PCR-RFLP) analysis was conducted to examine mutation with80samples of SD and100samples of normal control in Shandong Hans. We computed the genotype frequency of SD group and control group, studied the relation between the gene polymorphisms and SD.Results:in patients and control subjects,the frequencies of T allele were0.60and0.58, respectively. the frequencies of CC, CT, TT genotype of sudden deafness were0.13,0.54,0.33, x2was3.694,respectively. Meanwhile those of the control subjects were0.21,0.42,0.37, x2was0.147.Neither the genotype nor the allele frequency was significantly different between patients and control subjects. The sufferring SD risk of people with TT genotype was0.867than the other people(OR=0.867^CIO.041-0.932^P<0.05). the sufferring SD risk of people with CT genotype was1.605than the other people(OR=1.605, CI0.782-3.248、P>0.05).Conclusions:1. There was no distinctive difference between SD group and normal control group in genotype frequency and allele T frequency2. The C677T mutation of methylene tetrahydrofolate reductase gene is not a risk factor associated with sudden deafness (SD) in Shandong Hans, nevertheless,it is also Considered the influence of choose factors (quantity、age、sex、crowd), is worth following3. The allele T frequency is58%in normal Group of Shandong Hans,the Mutation rate is very high, wether the C677T mutation can be used as an independent genetic risk factors and an independent predictor of SD and other disease,is worth expanding the samples to deeper research...