Naoxintong Effects On The Brain Microvascular Endothelial Cells

BackgroundAtherosclerotic arteries (atherosclerosis) is a kind of common disease of serious harm to people’s health. In our country, especially in recent years, the incidence of the disease has a significantly increasing trend,the treatment methods of the research has become the top priori ty. When endothel ial cells damage and focal loss, which results in higher permeability of blood vessel walls, plasma lipoprotein into lining, then cause macrophage removal reaction and SMC hyperplasia of blood vessel walls,and a buildup of plaque,which is one of the important factors causing the AS. NaoXinTong possesses good effect in treating disease of heart head blood-vessel, but has yet to see its application in the oxidation of low density lipoprotein on the injury of endothel ial cells to repair the experiment.ObjectiveFrom6-keto-PGF1α and ET-1, the protein content of the point of view, Obse-ving NaoXinTong on brain microvascular endothelial cells and adhesion function of neutrophils, discusses NaoXinTong repair was oxidized low density lipoprotein damage mechanism of action of the human brain microvascular endothelial cells.Methods1the cultivation of the human brain microvascular endothelial cells (HBMEC): through the recovery of HBMEC, develop and extend the method, establish a stable cell experiment platform.2NaoXinTong drol iferation experiment on normal culture:through determined by MTT method to detect changes in cell activity, and then observed by HBMEC under normal culture after different concentration and time of NaoXinTong processing of cell proliferation, to experiment of safe, effective and non-toxic drug concentrations were determined.3oxidized low density lipoprotein of HBMEC cell damage experiment:through the cell activity changes determined by MTT method and observed by different concentration of oxidized low density lipoprotein cholesterol (hdl-c) after different time of HBMEC damage, caustic concentration to determine the choice made.4NaoXinTong from oxidized low density lipoprotein damage HBMEC protection experiment:through the cell activity changes determined by MTT method and application results from step2,3test NaoXinTong and oxidized low density lipoprotein concentrations, determination of NaoXinTong high,medium and low concentration determination drug group in different periods by oxidized low density lipoprotein cholesterol (hdl-c) of human brain microvascular endothel-ial cells injury protection.5NaoXinTong from oxidized low density lipoprotein damage of HBMEC ET-1,6-keto-PGF1α the influence of protein content determination by enzyme-linked immune adsorbent.NaoXinTong impact on these two cytokines protein content.Resul ts1after the recovery of HBMEC cell body hypertrophy, transparent, show lozenge or polygonal, nuclear small, round, the cell mono layer growth in paving stone, fits the characteristics of the endothelial cells.2NaoXinTong solution for24,48,72hours,under the concentration of187.5μg/ml, most promote HBMEC value-added, compared with normal control group (P<0.05).3In oxidized low density lipoprotein cholesterol(hdl-c) concentration to75、100、150μg/ml for24,48,72hours after the inhibition of cell growth, compared with normal control group,(P<0.05).4HBMEC role in oxidized low density lipoprotein cholesterol (hdl-c)24,48,72hours,activity decreased significantly, the difference com-pared with normal control group (P<0.05). While joining oxidized low density lipoprotein, join NaoXinTong solution,24,48,72hours,93.75、46.88、23.44、11.72、5.86μg/ml of the five group of oxidized low density lipoprotein damage is statistically significant (P<0.05).5Enzyme-linked immunosorbent determination ET-1in48,72hours, compared with normal group, damage group of ET-1levels increased significantly (P<0.05). Compared with damage group,NaoXinTong low concentration group, NaoXinTong concentration, NaoXinTong high concentration group of ET-1content decreased significantly (P<0.05). Enzymelinked immunosorbent determination6-keto-PGF1α48,72hours, compared with normal group, damage group6-keto-PGF1α content decreased sign ificantly(P<0.05). Compared with damage group, NaoXinTong low concentration group, NaoXinTong concentration, NaoXinTong high concentrate-ion group6-keto-PGF1α content increased significantly (P<0.05).Conclusion1in certain dose range,NaoXinTong can promote proliferation HBMEC under normal culture appropriately.2under the effect of a certain concentration range, the oxidized low density lipoprotein HBMEC may damage, but does not cause all of its apoptosis.3.NaoXinTong from oxidized low density lipoprotein damage HBMEC ET-1and6-keto-PGF1α have influence, may enhance the ET-1protein content, lower6-keto-PGF1α content, which can play a protective effect of HBMEC. NaoXinTong is verified by the study, to a certain dose range, from oxidized low density lipoprotein damage in vitro generation of HBMEC plays a protective role. Its action mechanism and promote the increment of HBMEC value-added, ET-1protein content, and6-keto-PGF1α inhibit the protein content.