The Effect Of Wheat Peptide On Red Blood Cell In Rats With Altitude Training And Its Mechanism

Objective:The study is to explore the intervention and interaction of aerobic exercises and wheat peptides on erythropoiesis in rats with altitude training. And to explore the mechanism of effect of hypoxemia and wheat peptide on erythropoiesis in rats with altitude training through the examination of oxygen-free radicals.Method:Choose Sprague-Dawley (SD) male rats,160-180g of each, are randomly divided into6groups for two-weeks raising:control group (group C. n=10), altitude control-group (group HC, n=10), exercise group (group E, n=10), exercise+wheat peptides group (group EW, n=10), altitude+exercise group (group HE, n=10), altitude+wheat peptides+exercise (group HEW, n=10). All rats are to be feed with normal diet according requirements and their body weight are measured every week. Meanwhile, the group HW and HEW added wheat peptide solution (dose for0.5g/kg·bw) after exercise training. Draw materials on next morning of the last exercise training and keeping fasting. Blood routine examination as well as erythrocyte SOD、GSH-Px activities、serum MDA and EPO contents are measured. The red cell morphology is observed under microscope.Results:(1)Compared with those of group C,the RBC count and HGB content in group HC rats increased significantly (P<0.01); the erythrocytes deformity rates in group E rats increased greatly (P<0.05). Based on the analysis of double-factor variance, Long-term hypoxia exposure can make the RBC count, HGB concentration and HCT of the rats with exercise training increase considerably (P<0.01), and the RBC count increased tremendously by adding wheat peptide (P<0.05), but there are no significant differences on the HGB and HCT (P>0.05). No remarkable interaction on the RBC, HGB, HCT of the rats by the combined hypoxia exposure with wheat peptides supplement (P>0.05)(2) Compared with that in group C, the serum EPO content and erythrocytes deformity rates in group HC rats has no significant difference (P<0.05), erythrocytes deformity rates in group E rats increased greatly (P<0.05). Through the analysis of double-factor variance, Hypoxia stimulates and wheat peptides supplement were not significant influence on serum EPO content in training rats (P>0.05). Hypoxia stimulation can make erythrocyte deformity rate in exercise training rats increased very significantly (P<0.01). and wheat peptides supplement can make erythrocyte deformity rate in exercise training rats significantly reduced (P<0.01), but the hypoxic stimulation combined with wheat peptides supplement had no significant interaction on erythrocyte deformity rate in training rats (P>0.05).(3) Compared with those of group C, the erythrocyte SOD and GSH-Px activities, MDA contents in group HC rats have no significant difference (P>0.05); the GSH-Px activity in group E rats increased greatly (P<0.05), and there are no special differences on the SOD and MDA (P>0.05) in group E rats. Through the analysis of double-factor variance, hypoxic stimulation can decrease s the erythrocyte SOD and GSH-Px activities (P<0.01) harply and increase the serum MDA concent (P<0.01) greatly in training rats. Wheat peptide supplement can cause erythrocyle SOD activity in training rats increase definitely (P<0.05) and there are no remarkable differences on the erythrocyle GSH-px activity and serum MAD concent. Combined hypoxic stimulation with wheat peptidesupplement has no conspicuous interaction on the erythrocyte SOD^GSH-Px activities and serum MDA concent (P>0.05)Conclusion:(1) Hypoxia stimulation can make RBC count, HGB content and HCT in exercise training rats increase very significantly, wheat peptides supplement can make increase RBC number in exercise training rats significantly. Therefore, a long-term altitude training can improve the blood function of oxygen transport.(2) Hypoxia stimulation can make erythrocyte deformity rate in exercise training rats increased significantly, while the wheat peptides supplement can make erythrocyte deformity rate decrease. Therefore, wheat peptides supplementary can alleviate red blood cell damage induced by altitude training.(3) Altitude training can make erythrocyte SOD and the GSH-Px activity decrease significantly, serum MDA content increased significantly, while the added wheat peplides supplement can make erythrocyte SOD activity in the altitude training rats increased significantly. Therefore, supplementary wheat peptides can reduce the generation of oxygen free radicals, reduce damage of red blood cell in the altitude training rats.