| "Succade" is a food of traditional and snack. It very popular, and is suitable for both young and old. In addition can be used as snacks or snack consumed directly, Succade can also be used in cakes, biscuits as an embellishment. Food is the first necessity of the people. Human health mostly depen ds on a healthy diet, mainly including raw material of food, the way of eating and drinking and so on. The diet from roots which is called the safety of the food ingredients. The freshness of raw material is the basic conditions for the protection of human health. Food preservation and corrosion have became the primary concern of producer during food processing. Food preservative and auxiliary reagent is based on the principle in the case does not affect the health of people, joining micro, side-effects, a smaller reagent to meet food preservation or improve chemomorphosis performance. These preservatives or additives has the standard of carefully selected and control. But some unscrupulous traders in order to meet the needs of consumers and attract consumers to buy their products, usage and use of unauthorized food additives. What is more, the use of unfood additives mixed with food, which causes confusion to the market and use of food additives.Therefore, to choose appropriate detection and identification methods of food additive security has become the focus attention. In this study, we chose sodium benzoate, potassium sorbate, sodium saccharin and acetyl potassium sulfonate as testing object. We applied high performance liquid chromatography to explore detection conditions of four kinds of additives in order to get a quick, accurate detection method. In addition to account for liquid feature of high performance liquid chromatography. Set of Gansu Province Dingxi and Lanzhou candied commercially available solid samples for the study, we compared the different pre-treatment methods. And the accelerated solvent extraction method were investigated by single factor and response surface optimization test. Then we come to the best pre-treatment process. According to the detection conditions of liquid chromatography which has been confirmed in this study, we investigated the additive components of candied fruits products from Gansu Province. The database of of candied fruits on four kinds of additives has been established. The results are as follows:(1) Welch materials xb-C18(4.6mm×250mm×5μm) was used and the mobile phase consisted of solution A(methanol) and solution B(ammonium acetate0.02mol/L) with gradient elution. The gradient program:10%methanol,90%ammonium acetate2min; 8%methanol,92%ammonium acetate4min;6%methanol,94%ammonium acetate15min.The flow rate was1.0ml/min, column temperature30℃, the injection volume was10μl. According to the precision test, the average relative standard deviation (RSD) was0.11%-1.98%, and the average recovery was91.67%-109.17%.(2) According to the recovery experiment, we compared with soak extraction, ultrasonic extraction and accelerated solvent extraction method of four additives in preserved samples. The result showed accelerated solvent extraction method has the characteristics of time saving and efficient extraction. The average recovery of four additives was98.7%-101.6%.(3) By using single factor test and response surface optimization test, the optimum process of accelerated solvent extraction was determined as:pressure of1020psi, temperature90℃, time6.5min. We repeated verification of test results to the aforementioned conditions for3times. Then we got the mean extraction rate of92.73%,107.14%,92.16%and93.81%respectively.(4) Comparison with existing national standards and methods of the literature, apparently analysis is the method of matrix complex, short analysis time, analysis of the level and reliable data and so on.We acquired chromatography database of Sodium benzoate, potassium sorbate, sodium saccharin and acetyl potassium sulfonate which were present in the sweetmeat Preliminarily. |
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