The Impact Of ZnO Nanoparticle On Aloe Protoplast

Along with the rapid development of global technology, nano-materials havebeen widely applied in various areas in human society and life. It has provideconsiderable economic benefits because of their unique physical and chemicalproperties. However, in recent years, scientists, while in studying the more and morebenefits that nano-materials can bring to us, are also aware of the potential risks toenvironment and human life that may arise from the wide application ofnano-materials. And in fact they do have caused some harm due to their specificnature. Many scientists confirmed that nano-particles have a certain degree ofpotential harm to animals, higher plants and the human body by experiments. Somestudies have shown that ZnO nanoparticles (ZnO NPs) have significant impact onmicro-organisms such as bacteria, animals such as rats, higher plants such as rape,radish, corn, and human body.But to the acknowledge of the author, we still lack enough researches on theimpact of nano-particles on plant cell protoplast. Therefore, in order to provide awider range of information for the studies of nanotoxicology, this paper manlyfocused on the effects of ZnO on the morphological properties of aloe protoplasts.Firs, I described the unique properties and applications of nano-materials and theresearch progress of nano-safety, especially the researches on the biosafety of ZnOnano-particles, because nano-ZnO is one of the most widely used nano-materials inscientific, technological fields, and in human life. Second, I discussed the sol-gelpreparation of ZnO nano-particles and enzymatic extraction of Aloe cell protoplast.Finally, I studied the effects of ZnO nano-particle concentration and cultivation timeon the morphological properties and the cell death rate. The statistical analysis of theresults showed that under certain ZnO NPs concentration, the death rate of aloeprotoplast increased with the cultivation time; and for a certain cultivation time, thedeath rate of aloe protoplast increased with the concentration of ZnO NPs. I alsoobserved the morphological evolution of single protoplast under the existence ofZnO NPs. These results provided some new information about the effects and risksof ZnO NPs at the cellular level.