The Establishment Of The Coating-chemiluminescence Sensor And Application In Detection Of Hydrogen Peroxide And Glucose

Hydrogen peroxide and glucose,which can dramatically affect the functions andmetabolism of organism’s cell, both are essential chemicals in organism and closely correlatedwith the health of human body. Thus, the determination of hydrogen peroxide and glucose inthe living environment are very important to environmental science, biology and clinicalmedicine. The present work developed a serial of methods for rapid, facile and precisedetermination of hydrogen peroxide and glucose. The details is listed as below:1. The chemiluminescence sensors were prepared by using C-18modified mesoporoussilica particles as substrate, then followed by immobilization of CPPO and rubrene on thesurface and inside of silica particle. The C-18can provide ahydrophobic environment tostabilize the CPPO and rubrene on the surface of silica particle, while the PF127can make theparticle hydrophilic, which facilitated the measurement of biospecimens. The use ofmesoporous silica particles provided a huge surface area for hydrogen peroxide to react withthe luminescence reagent, and thus improved the efficiency of resultant chemical sensor. Thehydrogen peroxide can be determined in wide concentration ranging （10-6mol/L—10-3mol/L）, where the intensity of luminescence was linearly correlated with the concentration ofhydrogen peroxide. The glucose can be detected in the concentration range of10-4mmol/L—5mmol/L with linear correlation of luminescence intensity vs concentration of glucose. It isideal for in vivo determination of glucose.The present chemical sensor overcame the poor bio-compatibility of peroxyoxalate ester.The luminescence reagents were enriched on the surface of silica particles, that could shortenthe distance of CPPO and rubrene, and then improve their energy conversion efficiency. Thus,the resultant chemical sensor possesses good bio-compatibility, high luminescence efficiency,high sensitivity and good durability.2. The CPPO and rubrene modified silica microparticles were prepared by coatingapproach. Then the glucose oxidase was immobilized on the surface of silica particles by thehydrophobic effect to result in the chemical sensor. The preliminary results showed that theresultant chemical sensor can be used to determine the content of glucose. The detailedinvestigation will be presented in further work.