Research On The Detetion Of PAEs And Antimony In Corn Beverage And Bottled Water

This work was supported by the China-Jilin Agricultural Product Quality and SafetyProject (Grant No.2011-Z67).In recent years, many food safety problems caused by plastic food packaging occurfrequently, which has drawed wide attention in society. Plastic products are synthesizedmainly by the plastic matrix resin, with additives added such as plasticizers, lubricants,antioxidants, flame retardants, etc., in order to meet industry needs and market needs. Forinstance, phthalate esters(PAEs) are often used in the plastics industry as plasticizers (ie,plasticizers) and antimony oxide as flame retardants. However, the presence of thesesubstances is of certain toxicity and safety risks for human. The illegal and excessive usein plastic food packaging may lead to migration during the direct contact process withfood, which often results in serious quality and safety issues for packaged food.This article briefly described the presence of the target phthalates substance, as wellas the existence of antimony used as flame retardants, and also introduced food safetyrisks and hazards they may cause. Plastic bottled corn juice, which is one of the mainproducts of corn industry in Jilin province, was selected as experiment samples togetherwith some other commercially available plastic bottled water. Gas chromatography-massspectrometry (GC-MS) method and hydride generation-atomic fluorescence spectrometry(HG-AFS) method were established separately for the detection of phthalate esters (PAEs)and Antimony (Sb), to evaluate their level in plastic bottled corn juice and some bottledwater chosen randomly in Changchun city.The main contents are as follows:（1）Gas chromatography-mass spectrometry (GC-MS) instrument was used toestablish experimental method to obtain four phthalates target substances’ linear relationship between concentration and peak area response through a comprehensiveexperiment. The four compounds are Dibutyl phthalate (DBP), Butyl benzyl phthalate(BBP), Di (2-ethylhexyl) ester (DEHP), Di-n-octyl phthalate (DNOP) a. Massspectrometry were used to analyze the results and obtain its fracture fragments data.Wherein the standard curves obtained four kinds of targets are: DBP, y=3×106x+246713, R=0.9982; BBP, y=556368x+48047, R=0.9984; DEHP, y=1×106x+116214, R=0.9996; DNOP, y=954823x+30847, R=0.9988. Thus, four kinds ofstandard curves fit well, with precision range of experiments0.64~0.98%, which weretrue and reliable. The precision of MS ion fragmentation information is less than5.56ss%,so the results were authentic. The method and linear relationship established,(i.e. thestandard curve) are for the qualitative and quantitative analysis of four target compoundsin corn juice and bottled water samples.In this part of the experiment, the sample’s pretreatment process was conducted usinga micro-injector purge gas extraction method. Based on condensation using semiconductortechnology and high-temperature heating, the sample and the extraction solvent were putin the syringe barrel driven by inert gas, for quantitative trace enrichment of a targetsubstance. The target allocation was completed between the extract phase and the gasphase, whereby the extraction is completed with the purification purpose of theexperiment. As it turned out, this experimental precision was of less than5%, and therecovery rate of85.73~108.88%, which meant method worked well and could be used.Four phthalates in corn juice and several bottled water samples were measured. Thenthe migration assay in extreme conditions was conducted, which was to detect the targetsubstances after the samples’ being processed under conditions of constant temperature40℃for10days, to complete the harm evaluation. In this two parts of experiments, BBP,DNOP were not detected. While the presence of small amounts of DBP, DEHP weredetected in higher frequencies. The overall PAEs concentrations were lower than0.030047mg/L. Experimental results showed that, migration of DEHP into food was of ahigher risk. During storage process of the plastic packaged foods, the temperature had an important role in the migration of phthalates.（2）A hydride generation-atomic fluorescence spectrometry method wasestablished to determine Antimony in plastic packaging of corn juice and several bottledwater samples. The standard curve was If=5.15C+305.5, with the regression coefficientR2=0.9960, to meet the needs of this experiment. Processing of the plastic packagingsamples was conducted using food simulants’ immersion method, with4%acetic acidsolution60℃soaking for2h. The experimental results showed that all the20sampleswere with a lower level of Sb, being not excessive.In the first part of the experiment, four phthalates and their migration were studied; inthe second part, mainly the Sb levels of these plastic bottles samples were determined. Theresearch on the detection of plastic phthalates and antimony oxide in bottled water, play acertain role in the assessment and guidance for the safety of food packaging.