| China is a high incidence of anemia disease in the world, therefore, enriching blood functional food has great market demand. In recent years, with the development of China’s functional food industry, the products of improving the nutritional anemia functional food have increaced. In terms of the dosage forms, oral liquid has broad application because of the advantages of absorb easy, action fast and stablity. However, in terms of raw materials, enriching blood oral health foods of red peanut skins as a raw material have not been published in the paper yet. In this thesis, the "four red" peanut in northeast specialty was used as the main raw material, adding NaFeEDTA, jujube concentrate and other auxiliary materials, and we has developed a kind of functional oral improving iron deficiency anemia. The indicators of sensory, physical and health were formulated, and finally the role of improving iron deficiency anemia was determined through animal experiments.(1) The preparation process research of red peanut skin extract:The ethanol extraction method was used to extract the active ingredient in red peanut skin(total flavonoids). Based on the single factor experiments, response surface design software was used for the further analysis. And the optimal parameters were ethanol concentration of80%, liquid-solid ratio of25:1(mL/g), extraction temperature of62℃and extraction time of2h. The yield of total flavonoids in red peanut skins was16.45%under the conditions.(2) The formulation and process parameters determine of enriching blood oral liquidof red peanut skin:Firstly, the jujube was processed using complex enzymatic method. The different ratios of pectinase and cellulase were compared, and the optimal ratio found was1:1. The optimal process parameters of jujube concentrated solution were studied by orthogonal experiment. Namely complex enzyme dosage was0.04%, reaction temperature was50℃, hydrolysis time was80min, under the process conditions, total sugar content of jujube juice was up to9.56%; Then the effect of ferrous lactate, ferrous gluconate and NaFeEDTA on the taste of oral was compared. The results showed that NaFeEDTA had the most minimal impact on the taste and the most appropriate concentration of Fe was25mg/100mL, namely to add0.19%of NaFeEDTA as the iron nutrition enhancer; Finally, the best formulation of enriching blood oral of red peanut skin was studied by orthogonal experiment. They were peanut skin extract of0.15%, NaFeEDTA of0.19%, jujube concentrate of20%, sugar of6%and lemon acid of0.08%. Then the product was sterilized in water bath of85℃for30min. Under the conditions, the content of total flavonoids was greater than or equal40mg/100mL, the content of iron was20-30mg/100mL, and all indicators of the product were in line with the quality standards.(3) Functional evaluation of enriching blood oral liquid of red peanut skin:To further validate the product’s efficacy of improving the iron deficiency anemia, SD rats were selected to test. The three indicators of body weight, hemoglobin (Hb), erythrocyte protoporphyrin (FEP) were measured. The experimental results showed that the produce could increase the weight of the animals tested significantly, and compared with the low-iron group, the produce elevated Hb concentration and reduced FEP concentration in the blood of animals significantly, indicating that the product could improve the iron deficiency anemia. |
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