Effect Of Different Addition Of Cu,Mn,Cr, And Se On Prodction Performance, Serum Index And Duodenum Development In Weaned Rex Rabbits

In this experiment, we used L16(45) design protocol, studied the effect of additionamount of copper, manganese, chromium, and selenium to weaned Rex Rabbit’s productionperformance, seroimmunity, antioxidase activity and intestinal development of the duodenum.Four trace elements were added as forms of copper sulfate pentahydrate, manganese sulfatemonohydrate, chromium chloride hexahydrate and sodium selenite, respectively.We chose192Rex Rabbits which were weaned in30days. Rabbits were randomlydivided into16treatments with four replicates of three rabbits each. Test was divided intoseven days pre-feeding period, a formal period of60days. Treatment groups were added tothe basal diet based on combinations of four different levels of copper, manganese, chromium,and selenium of four trace elements. As for the control treatments, we added0mg/kg ofcopper,0mg/kg of manganese,0mg/kg of chromium and0mg/kg of selenium. Productionperformance, seroimmunity indicators, antioxygen indicators, length and crypt depth ofduodenal intestinal villus of all the treatments were observed and recorded.The results were as follows:1. Production performance:①There were significant effects (P <0.05) of addtionalcombination of copper, manganese, chromium, and selenium on the average daily gain andfeed weight ratio of the entire stages, in the meanwhile, there was a significant effect (P<0.01)of addtional combination of copper, manganese, chromium, and selenium on the average dailyfeed intake of the entire stages.②Compared to Mn0treatment， total daily gain of Mn35，Mn70and Mn105treatment were decreased by4.51%,9.35%,5.95%respectively.③Highlevel of manganese and high level of chromium led to reduced feed intake.④The treatment ofCu0+Mn0+Cr0+Se0was the best.2. Aspartate aminotransferase and alanine aminotransferase in Serum:①There was asignificant effect (P <0.05) of on addtional combination of copper, manganese, chromium,and selenium on activities of aspartate aminotransferase, but there was on significant effect(P>0.05) on activities of alanine aminotransferase in serum.②The treatment ofCu225+Mn35+Cr10+Se0was the best.③There was a strong tolerance to high levels ofcopper, manganese, chromium and selenium on liver of30d weaned Rex rabbit.3. Hormones in Serum:①There were on significant effect (P>0.05) of on addtional combination of copper, manganese, chromium, and selenium on free triiodothyronine andgrowth hormone.②The treatments of Cu0+Mn70+Cr10+Se0.3and Cu75+Mn70+Cr15+Se0were best.4. Immunoglobulin in Serum:①There were a significant effect (P <0.05) of addtionalcombination of copper, manganese, chromium, and selenium on the content of IgA and IgM,but there was no significant effect (P>0.05) on the content of IgG in serum.②Fromperspective of the content of IgA、 IgM and IgG, Cu150+Mn105+Cr5+Se0、Cu0+Mn35+Cr5+Se0.2and Cu150+Mn70+Cr0+Se0.2were best respectively.5. Antioxidase activity in Serum:①There was a significant effect (P <0.05) of addtionalcombination of copper, manganese, chromium, and selenium on activities of glutathioneperoxidase, but there were on significat effect (P>0.05) on activities of ceruloplasmin, copperand zinc superoxide dismutase, manganese superoxide dismutase and total superoxidedismutase(T-SOD).②From perspective of activities of glutathione peroxidase、copper andzinc superoxide dismutase, manganese superoxide dismutase and ceruloplasmin,Cu225+Mn105+Cr0+Se0.3、 Cu0+Mn105+Cr15+Se0.4、 Cu75+Mn105+Cr10+Se0.2、Cu75+Mn70+Cr15+Se0were best.6. Duodenal intestinal development:①There were no significant effect (P>0.05) ofaddtional combination of copper, manganese, chromium, and selenium on duodenal villilength, crypt depth and the ratio of villus length and crypt depth.②The treatment ofCu75+Mn35+Cr0+Se0.4was the best.