The Preparation Of Emulsifier And Its Application In Broilers Diets With Different Oil And Fat

This study was aimed to the preparation of emulsifiers by compared with emulsifiers on the effect of the emulsification stability of oils ands fat, lipase activity in vitro, and its effect on the growth performance, lipid metabolism, nutrient utilization and the digestive enzyme activity of broilers. The study included four parts:Experiment1, this experiment was conducted to determine the hydrophile lipophile balance (HLB) of four emulsifiers and two oils and fats, four kinds of compound emulsifier were fomulated based on HLB, and the emulsification stability of emulsifiers on soybean oil (SO) and lard (LA) was compared. The result showed that the HLB of SO and LA were6.0and5.0, and HLB of sucrrose esters of fatty acids, lysolecithin, tween80, span60were11.0,6.0,15.0and4.3, respectively. Furthermore, emulsifier with better emulsification effect was considered.Experiment2, this experiment was conducted to evaluate the effect of emusifier on lipase activity by simulating broiler gastrointestnal environmental conditions in vitro. The result showed that emulsifier could increase lipase activity.Experiment3, the objective of this experiment was to determine the effect of emulsifier on the growth performance, lipid metabolism of Arbor Acres (AA) broilers birds. A2×2factorial arrangement of treatments was used in the experiment,320one-day-old AA broilers were randomly assigned to four dietary treatments, and each treatment had8replicates of10broilers each. The broilers were given one of four diets:(1) soybean (SO) basal diet;(2) lard (LA) basal diet;(3) SO basal diet plus0.15%emulsifiers;(4) LA basal diet plus0.15%emulsifiers. The results showed as follows:Oils and fats, had no significant influence on the growth performance of broilers in the each phase (P>0.05), emulsifier supplementation decreased feed/gain ratio (F/G) of broilers in the starter phase (P<0.05), there was no interaction between oils and fats and emulsifier for the growth performance on broilers in the each phase (P>0.05). Compared with LA diet, SO diet and emulsifier supplementation obviously reduced triglyceride (TG) level in serum of broilers at7d (P=0.052) and42d (P<0.05), there was no interaction between fat and emulsifier for TG level in serum of broilers in the each phase (P>0.05), SO diet obviously increased high density lipoprotein cholesterol (HDL-C) level (P<0.05) and reduced low density lipoprotein cholesterol (LDL-C) level (P<0.05) in serum of broilers at7d and14d, and increased the ratio of HDL-C and LDL-C in serum of broilers at7d (P<0.05). Emulsifier supplementation obviously reduced total cholesterol (TC) level in serum of broilers at14d (P<0.01), increased HDL-C level in serum of broilers at7d (P<0.01) and14d (P<0.05), and increased the ratio of HDL-C and LDL-C in serum of broilers at7d (P<0.01) and21d (P<0.05), there was an interaction between fat and emulsifier for TC level in serum of broilers at14d (P<0.05). SO diet obviously increased lipoprotein lipase (LPL)(P<0.01) and hepatic lipase (HL)(P<0.05) activity in liver of broilers at7d, emulsifier supplementation obviously increased HL activity in liver of broilers at7d (P<0.01). Besides reducing LPL activity at7day of broilers (P>0.05), emulsifier supplementation increased LPL and total lipase (TL) activity in liver of broilers in the each phase (P<0.05), there was interaction between fat and emulsifier for HL and TL activity in liver of broilers at14d (P<0.05) and42d (P=0.054), and for LPL activity in liver of broilers at21d (P=0.059) and42d (P<0.05).Experiment4, the objective of this experiment was to determine the effect of emulsifier addition on nutrient utilization and the digestive enzyme activity of broilers. The trial materials and methods were the same as those in experiment3. The results as follows:Compared with LA diet, SO diet obviously increased nutrient utilization of broilers during8-10d and15-17d (P<0.05), emulsifier supplementation obviously increased utilization of crude fat (EE) of broilers during8-10d (P<0.05) and15-17d (P=0.053), and increased utilization of crude protein (CP) of broilers during15-17d (P<0.05), there was interaction between fat and emulsifier for AME of broilers during8-10d and15-17d (P<0.05). Fat had no significant influence on the digestive enzyme activity in pancreas of broilers in the each phase (P>0.05), emulsifier supplementation obviously increased trypsin activity.in pancreas of broilers in the each phase (P<0.05), besides trypsin activity at14day of broilers (P>0.05), and increased amylase activity in pancreas of broilers at21d and42d (P<0.01), there was an interaction between fat and emulsifier for trypsin activity in pancreas of broilers at21d (P<0.05). SO diet and emulsifier supplementation obviously increased protease (P=0.086) and trypsin (P=0.092) activity in digesta of broilers at42d, there was no interaction between fat and emulsifier for the digestive enzyme activity in digesta of broilers in the each phase (P>0.05).These results implied that emulsifier could improve the growth performance and lipid metabolism, increase the digestive enzymes activity in broilers, compared with SO, LA diet with emulsifier could better improved the growth performance and other facts in broilers.