| Lentinula edodes is popular with people because of its rich nutritional and medicinal value. Lentinula edodes has a very important position in the world,now it’s the second largest artificial cultivation mushroom, the yield is only less than Agaricus bisporus.The traditional cultivation materials of Lentinula eddoes is hardwood sawdust, As the industry’s rapid development and the resource of broad-leaved forest continual depletion,we need to actively explore other resources to replace the sawdust. This project is to study the materials of citrus branch chips, chestnut shell powder and bamboo chips in the cultivation of Lentinula edodes, to compare different medium formula and cultivation methods, and to research the influenceofseveral kinds of tree and fruit tree residues to hypha growth,yield,biological efficiency and commodity character. We Obtained the optimal formula, and laid the foundation of tree and fruit tree residues in the cultivation of Lentinula edodes.In the test of shiitake mushroom cultivation by citrus branch chips as main medium, citrus branch chips and hardwood sawdust were mixed in different ratios to make different formulations. the contents of citrus branch chips were20%,40%,60%,80%and respectively the contents of hardwood sawdust were60%,40%,20%,0,the rest was thesupplementary,named A,B,C,D. The formula of hardwood sawdust as the only ingredients was regarded as control(CK).In the five formulas, Xiaxiang18and Qiuzai number7were the selected strains which shiitake cultivation by cultivation with the casing soil covering on cultural bags in summer and cultivation with the cultural bags placed on shelves in greenhouses in autumn respectively.The results showed that in the two methods of cultivation, growth rate of A,B,C,D were faster than CK.Spawn run time and fruitbodying of B,C were faster than CK. Yield and biological efficiency of A,B,C were higher than CK.In morphological characteristics of fruitbody,all formulations were good besides formula D.In the test of shiitake mushroom cultivation by chestnut shell powder as main medium,Xiaxiang18was the selected strains which shiitake cultivation in the summer.Chestnut shell powder were25%,50%,80%respectively and mixed with hardwood sawdust,in turn be numbers for A,B,C. The formula of hardwood sawdust as the only ingredients is regarded as control(CK). It turned out that growth rate of B,C were slower and could not form fruitbody.Yieldof A was lower than CK,too. Qiuzai number7was the selected strains which shiitake cultivation in the autumn. The formula of hardwood sawdust as the only ingredientswas regarded as control(CK). Chestnut shell powder and hardwood sawdust were mixed in different ratiosto make of different formulations, and they were numbered D,E,F,G. It turned out that growth rate of E,G were faster than or close to CK,butfruitbodying were later. Yield and biological efficiency of CK were the highest level.All morphological characteristics of fruitbody of formulations were superior or close to CK.In the test of shiitake mushroom cultivation by bamboo chips as main medium, L109was the selected strains which shiitake cultivation by cultivation with the cultural bags standed on the ground in greenhouses after plastic bags removal in autumn. Bamboo chips and hardwood sawdust were mixed in different ratiosto make of different formulations,and the contents of bamboo chips were20%,40%,60%,80%and respectively the contents of hardwood sawdust were60%,40%,20%,0,the rest is thesupplementary,named A,B,C,D. The formula of hardwood sawdust as the only ingredients wasregarded as control(CK).The results indicated that growth rate of A,B,C,D were faster than CK, fruitbodying were close to CK. Yield and biological efficiency of formula B were highest,an formula D were lowest.In morphological characteristics of fruitbody,all formulations were good and close to CK besides formula D.In conclusion, citrus branch chips, chestnut shell powder and bamboo chips could be respectively used to cultivate shiitake mushroom in different methods. Separately, when the proportion of citrus branch chips within60%, the proportion of chestnut shell powder within25%, the proportion of bamboo chips within60%, yield and biological efficiency were higher, morphological characteristics of fruitbody were better.Among,yield and biological efficiencyare highest, appearance of fruitbody were excellent if cultivation of shiitake mushroom by citrus branch chips40%, sawdust40%, supplementary20%in cultivation with the cultural bags placed on shelves in greenhouses in autumn,and chestnut shell powder20%, sawdust60%, supplementary20%in cultivation with the cultural bags placed on shelves in greenhouses in autumn,and bamboo chips40%, sawdust40%, supplementary20%in cultivation with the cultural bags standed on the ground in greenhouses after plastic bags removal in autumn.By correlation analysis between the biological character of cultivation of shiitake mushroom, we found that different medium had effect on biological character.And itwas the first prerequisite of appropriate medium what mycelia can grow well. Fruitbodying had a significant negative influence on biological efficiency of fruitbody. |
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