| Copper is an essential trace element of animals, can effectively maintain the stability of the internal environment, and is closely related with hematopoiesis, metabolism, growth, reproduction and other important life activities. While copper is also involved in comprising core components or cofactors of a variety of antioxidant enzymes of the important antioxidant system in the animals’body, it can timely clean-up of free radicals in the body, to ensure the growth and decline of homeostasis of the body of free radicals, reduce peroxides damage to the body. Nano-copper in a number of pilot studies showing that, compared with other copper sources has obvious advantages, not only add less, and also high absorption and utilization.This subject was conducted to determine the content in the blood serum of many antioxidant indicators on weaned Piglets of health dual hybrid (D IV x Large White pigs). Aimed at study the influence of serum antioxidant capacity on weaned piglets with different doses, different forms of copper,discussion on the intrinsic mechanism of copper, which play a role of inhibition of oxidation and reduction in the organism,as well as provide the experimental basis for the practical applications of nano-copper feed additive.The experiment selects28piglets of health,weight similar, Dual hybrid(D IV x Large White) given birth by3-5tires, litter8-10first Parity sows in the Huazhong Agricultural University experimental farm,weaned at21days of age,and were randomly divided into three groups:control group, copper sulfate group and copper oxide nanoparticles group. The control group was fed with basal diet5mg/kg copper, copper sulfate groups were fed the diets containing50mg/kg,100mg/kg,200mg/kg copper sulfate based diets, nanometer copper oxide groups were fed with25mg/kg,50mg/kg,200mg/kg nanometer copper oxide based diets,4piglets in each group continuous feeding14days,picks the blood on the anterior vena cava On the28day,35day age, prepares serum, then determines the content of serum antioxidant capacity indicators which contains copper-zinc superoxide dismutase (CuZn-SOD), ceruloplasmin (CP), malondialdehyde (MDA), myeloperoxidase (MPO), total antioxidant capacity (T-AOC), peroxidase (POD), nitric oxide (NO), nitric oxide synthase (NOS), hydrogen peroxide (H2O2), inhibition of hydroxyl radical (CIHR) etc.The results showed thatrwith the concentration of copper additive in the piglet diets increasing, SOD activity is also showing a trend of gradually increased; CP control group compared with copper sulfate group,25mg/kg and50mg/kg nano--copper oxide group, there was no significant differences (P＞0.05), while with200mg/kg nano-copper oxide group,there was a significant difference (P<0.05). However, with the increase of the concentration of copper, the activity of CP is also increasing; MDA control group compared with copper sulfate group,25mg/kg nanometer copper oxide group,50mg/kg nanometer copper oxide group,the content of MDA has a significant different (P <0.05),and50.5%lower in the experimental group than the control group, MDA content with increasing dietary copper concentration decreased, and the MDA content reduced6.8%average of second week than the first week; there was no significant difference in the control group, copper sulfate test group and nano-copper oxide test group of MPO (P0.05), as the copper concentration increases, the MPO activity has no qualitative changes, therefore presumed that the copper concentration of the additive has no direct effect on MPO activity; T-AOC test group compared with100mg/kg copper sulfate group,200mg/kg copper sulfate group,25mg/kg nano-copper oxide group,50mg/kg nano-copper oxide group,there were both a significant difference (P<0.05), and with copper content increases, the total antioxidant capacity of the blood is also on the rise; the POD test group with the control group showed a significant difference (P<0.05), and the peroxidase activity of the test group was21.9%higher on average than the control group, which indicated that the POD vitality and content of dietary copper concentration was positively correlated; on the NO and NOS trial,beside the200mg/kg nano-copper oxide group has no significant difference with the control group (P＜0.05), the other test group were statistically significant difference with the control group(p<0.05), and the NO content and NOS activity showed a gradual decrease with increasing dietary copper concentration trend;On the H2O2trial,in addition to no significant difference between25mg/kg nanometer copper oxide group and the control group (P□0.05), there were significant differences between he rest of the experimental group and the control group (P <0.05), the test group was28.9%lower on average than the control group, and H2O2content gradually decreased with copper content increasing;CIHR blank control group and the test group has differences significantly (P<0.05), and copper sulfate group was8.9%higher on average than the control group, nano-copper oxide group was32%higher on average than the control group, and as the copper content increases, the ability of serum inhibited hydroxyl free radicals are gradually increasing,while the difference between the same concentration of copper sulfate group and nano-copper oxide group was also significant (P<0.05),and nanometer copper oxide group was39.6%higher than copper sulfate group in the50mg/kg test group,and the200mg/kg trial group,10.8% above the nano-copper oxide than the copper sulfate.These results suggest that:the nano-copper oxide may be more conducive to the body’s absorption and utilization than copper sulfate; the200mg/kg nano-copper oxide have a certain damage to body; Add feed concentration of nano-copper oxide should be controlled between50mg/kg and200mg/kg; feed copper concentration increases can improve production performance by strengthening the body’s antioxidant capacity. |
PDF Full Text Request