Dietary Application Of Compound Enzyme Preparation To Yellow Catffsh (pelteobagrus Fulvidraco) Feed

A feeding trial was conducted to investigate the effects of aquatic compound enzymepreparation (protease, lipase, amylase and pectinase) supplemented in the diets for Yellow catfish(Pelteobagrus fulvidraco) on the growth performance, body composition, digestive enzymesactivities, serum biochemical and nonspecific immune related indicators. The results are asfollows:1. The weight gain rate (WG), specific growth rate (SGR) and feed intake (FI) of Yellowcatfish showed an increasing tendency with compound enzyme preparation supplementation, andWG, SGR and FI in400mg/kg group were significantly higher than those in control group (P <0.05), increasing by15.4%,7.5%and20.3%, respectively. There was no difference in feedefficiency (FE) among dietary treatments (P>0.05).2. There was a significant increase on viscera index (VSI) and condition factor (CF) inYellow catfish fed with compound enzyme preparation compared to the control (P <0.05). CFreached the highest in fish fed the diet containing400mg/kg of compound enzyme preparation.VSI showed the highest value in200mg/kg group, and the value was significant higher than thatof control group (P <0.05). There were no differences in whole body crude protein, crude lipid,ash and moisture among treatments (P>0.05).3. Significant increased effects of compound enzyme preparation supplementation on theactivities of protease in stomach and hepatopancreas and amylase activities in stomach, intestinesand hepatopancreas were observed (P<0.05). The activities of protease and amylase in stomachand protease in intestine showed an increasing tendency (P<0.05) with the increase in dietarycompound enzyme preparation supplementation. The activities of protease and amylase inhepatopancreas decreased with the increase in dietary compound enzyme preparationsupplementation (P<0.05).4. The contents of CHO, TG and GLU in serum increased with the increase in dietarycompound enzyme preparation supplementation (P>0.05), while the serum UA contentsignificantly decreased (P<0.05). The activities of serum ALT and AST showed an increasingtendency with the increase in dietary compound enzyme preparation supplementation, and thefish fed the diet at400mg/kg or200mg/kg had the highest value, and was significantly higherthan that of the control group (P<0.05).5. The content of TP and activity of LZM in serum firstly increased then decreased with theincrease in dietary compound enzyme preparation supplementation, reaching the maximum at 100~200mg/kg, and the values of them were significantly higher than those of the control group(P<0.05). The content of MDA and activity of SOD in serum did not differ across all thetreatments (P>0.05).Based upon the above results, the optimal dose of compound enzyme preparation in feed forYellow catfish is400mg/kg.