Technique On Cutting And Tissue Culture Of Dendrobenthamia Angustata Var. Wuyishanensis

Dendrobenthamia angustata var. wuyishanensis is a local plant that belongs to Dendrobenthamia. It has been subjected to heacy collection from the wilds due to ever increasing demand This paper sicussion about the propagation of the species using in vitro techniques. Rearch the propagated system and applying them to the urban greening have important theory and practice meaning for protecting this species effective in another place and enriching the kinds and views of ornamental plants.This paper studied the propagated system of Dendrobenthamia angustata var. wuyishanensis. systematically from the three aspects of cuttage and tissue culture. The results showed that:1、The cuttage rooting rates of annotators branch are higher than the perennial branch and the rooting rate can increase40%-50%.2、During the eight cuttage mediums, vermiculite on the experiment in the cuttage is the best, and follow by yellow sand is the second one.3、Rooting accelerators effect on rooting rates significantly.The rooting rate of the optimum handled combination is100ppm of ABT with30min.The rooting rate reached90%.4、In the sterilization process of leaves and stems of tissue culture process, the soak period of mercury dichloride which the concentration was0.1%played an important role. The best timing for stems was10minutes and the sterility rate could reach77.5%. Prevention and cure contrast test was conducted in order to solve the problem of severe pollution of bacteria in tissue culture for D. capitata var. emeinsis. Using sterile aqueous solution of penicillin with7million units per liter and30min to could effectively control the pollution of tissue culture for Dendrobenthamia angustata var. wuyishanensis.It is necessary to add some activated charcoal (4g/L) into medium. The effects of activated charcoal could be attributed to providing a dark environment in the medium, adsorption of certain inhibitory substances in medium.5、When we use the buds as culture material to induce the organogenesis in vitro, the optimal medium for inducing buds is:MS+6-BA0.5mg/L+GA1.5mg/L.Plantlets could grow healthy on this medium. The optimal medium for the multiplication of the buds is MS+6-BA0.5mg/L+ZT0.5mg/L。...